PMID: 9653105Jul 8, 1998Paper

Genes from mycoparasitic fungi as a source for improving plant resistance to fungal pathogens

Proceedings of the National Academy of Sciences of the United States of America
Matteo LoritoI G Fernandez

Abstract

Disease resistance in transgenic plants has been improved, for the first time, by the insertion of a gene from a biocontrol fungus. The gene encoding a strongly antifungal endochitinase from the mycoparasitic fungus Trichoderma harzianum was transferred to tobacco and potato. High expression levels of the fungal gene were obtained in different plant tissues, which had no visible effect on plant growth and development. Substantial differences in endochitinase activity were detected among transformants. Selected transgenic lines were highly tolerant or completely resistant to the foliar pathogens Alternaria alternata, A. solani, Botrytis cinerea, and the soilborne pathogen Rhizoctonia solani. The high level and the broad spectrum of resistance obtained with a single chitinase gene from Trichoderma overcome the limited efficacy of transgenic expression in plants of chitinase genes from plants and bacteria. These results demonstrate a rich source of genes from biocontrol fungi that can be used to control diseases in plants.

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Citations

May 2, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Patricia Ann OkubaraKimberlee K Kidwell
May 1, 2007·Applied Microbiology and Biotechnology·Yanhua FanYan Pei
May 1, 2012·Applied Microbiology and Biotechnology·Lakshmi PrasannaSigrid Gåseidnes
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Mar 24, 2010·Proceedings of the National Academy of Sciences of the United States of America·Karl-Heinz KogelUwe Sonnewald
Oct 24, 2008·Phytopathology·Gary E Harman
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Jan 11, 2005·Applied and Environmental Microbiology·Weiguo FangYan Pei
Mar 5, 2005·Applied and Environmental Microbiology·Brian BoyleArmand Séguin

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