PMID: 3877933Nov 1, 1985Paper

Genes from plasmid pKM101 in Haemophilus influenzae: separation of functions of mucA and mucB

Proceedings of the National Academy of Sciences of the United States of America
M Balganesh, J K Setlow

Abstract

Haemophilus influenzae, normally not mutable by UV, became UV mutable with a recombinant plasmid insertion. A 7.8-kilobase-pair (kbp) fragment of the plasmid pKM101 containing the mucA and mucB genes was ligated to the shuttle vector pDM2, and a Rec- strain of H. influenzae was transformed with the ligated mixture. All of the transformants, unlike the parent Rec- strain, were resistant to UV, could carry out postreplication repair and Weigle reactivation, showed greatly increased spontaneous mutation, and contained a plasmid carrying an insert of only 1.2 rather than 7.8 kbp. This plasmid in a umuC mutant strain of Escherichia coli complemented a pKM101 derivative lacking mucA function but with an intact mucB gene, although there was no complementation with a mucA+ mucB- plasmid, suggesting that the newly constructed plasmid coded for the mucA protein; this is in accord with the restriction analysis and hybridization between the plasmid and a probe containing all of the mucA gene but only a small fraction of mucB. When one of the H. influenzae Rec- transformants lost the plasmid, the resistance to UV was retained but the high spontaneous mutation and UV mutability were not. The fact that there was hybridization between the chro...Continue Reading

References

Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J McCannB N Ames
Jan 2, 1979·Molecular & General Genetics : MGG·K E Mortelmans, B A Stocker
Apr 17, 1979·Molecular & General Genetics : MGG·G C Walker, P P Dobson
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Jul 21, 1972·Journal of Molecular Biology·J K SetlowR F Kimball
Dec 30, 1963·Zeitschrift für Vererbungslehre·W HARM, C S RUPERT

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Citations

Mar 1, 1991·Mutation Research·F MisuracaS Grimaudo
Jun 19, 2001·Mutation Research·J K SetlowE Cabrera-Juárez
Jan 1, 1987·Environmental and Molecular Mutagenesis·R K Elespuru
Jul 1, 1989·Journal of Bacteriology·C M Smith, E Eisenstadt
Oct 1, 1990·Molecular and Cellular Biology·M Tosu, H Tanooka
Sep 1, 1990·Journal of Bacteriology·C M SmithE Eisenstadt
Apr 1, 1988·Journal of Bacteriology·S G SedgwickF Malik

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