Genes Indicative of Zoonotic and Swine Pathogens Are Persistent in Stream Water and Sediment following a Swine Manure Spill

Applied and Environmental Microbiology
Sheridan K HaackWilliam T Foreman

Abstract

Manure spills into streams are relatively frequent, but no studies have characterized stream contamination with zoonotic and veterinary pathogens, or fecal chemicals, following a spill. We tested stream water and sediment over 25 days and downstream for 7.6 km for the following: fecal indicator bacteria (FIB), the fecal indicator chemicals cholesterol and coprostanol, 20 genes for zoonotic and swine-specific bacterial pathogens by presence/absence PCR for viable cells, one swine-specific Escherichia coli toxin gene (STII gene) by quantitative PCR (qPCR), and nine human and animal viruses by qPCR or reverse transcription-qPCR. Twelve days postspill, and 4.2 km downstream, water concentrations of FIB, cholesterol, and coprostanol were 1 to 2 orders of magnitude greater than those detected before, or above, the spill, and genes indicating viable zoonotic or swine-infectious Escherichia coli were detected in water or sediment. STII gene levels increased from undetectable before or above the spill to 10(5) copies/100 ml of water 12 days postspill. Thirteen of 14 water (8/9 sediment) samples had viable STII-carrying cells postspill. Eighteen days postspill, porcine adenovirus and teschovirus were detected 5.6 km downstream. FIB conce...Continue Reading

References

Dec 18, 2001·Journal of Virological Methods·Shigeyuki KojimaKazuhiko Katayama
Oct 9, 2003·Applied and Environmental Microbiology·Miguel Angel Jiménez-ClaveroVictoria Ley
Mar 10, 2004·Applied and Environmental Microbiology·Carlos Maluquer de MotesRosina Girones
Sep 24, 2004·Bioresource Technology·Fiona A NicholsonBrian J Chambers
Sep 29, 2004·American Journal of Public Health·David Osterberg, David Wallinga
Dec 8, 2004·Journal of Clinical Microbiology·Florence DepardieuPatrice Courvalin
Mar 7, 2006·Applied and Environmental Microbiology·Marilyn SouleDouglas R Call
Mar 27, 2007·Environmental Health Perspectives·Joann BurkholderMichael Wichman
Jan 6, 2010·Journal of Environmental Quality·Shalamar D ArmstrongLayi Adeola
Jan 23, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Barbara RichterHerbert Weissenböck
Apr 10, 2012·PloS One·Aparna LalMichael G Baker
Dec 5, 2012·The Journal of Antimicrobial Chemotherapy·Vincent Cattoir, Roland Leclercq
Sep 28, 2013·International Journal of Environmental Research and Public Health·Danielle M Yugo, Xiang-Jin Meng
Oct 1, 2013·Environmental Science & Technology·Sheridan K HaackHeather E Johnson
May 6, 2014·Water Research·Jessica J EichmillerRandall E Hicks

❮ Previous
Next ❯

Citations

Jun 20, 2016·The Science of the Total Environment·Carrie E GivensSusan K Spencer
Oct 11, 2017·Journal of Environmental Quality·Kristen E GibsonGiselle Almeida
May 16, 2020·Frontiers in Bioengineering and Biotechnology·Deisi Cristina TápparoGislaine Fongaro
Jan 11, 2019·Frontiers in Microbiology·Elizabeth L RiekeAdina C Howe
Jan 3, 2018·Journal of Environmental Quality·Robert S DunganDavid L Bjorneberg
May 4, 2016·The Science of the Total Environment·Sheridan K HaackWilliam T Foreman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.