Genes involved in control of galactose uptake in Lactobacillus brevis and reconstitution of the regulatory system in Bacillus subtilis

Journal of Bacteriology
G M DjordjevicM H Saier

Abstract

The heterofermentative lactic acid bacterium Lactobacillus brevis transports galactose and the nonmetabolizable galactose analogue thiomethyl-beta-galactoside (TMG) by a permease-catalyzed sugar:H(+) symport mechanism. Addition of glucose to L. brevis cells loaded with [(14)C]TMG promotes efflux and prevents accumulation of the galactoside, probably by converting the proton symporter into a uniporter. Such a process manifests itself physiologically in phenomena termed inducer expulsion and exclusion. Previous evidence suggested a direct allosteric mechanism whereby the phosphocarrier protein, HPr, phosphorylated at serine-46 [HPr(Ser-P)], binds to the galactose:H(+) symporter to uncouple sugar transport from proton symport. To elucidate the molecular mechanism of inducer control in L. brevis, we have cloned the genes encoding the HPr(Ser) kinase, HPr, enzyme I, and the galactose:H(+) symporter. The sequences of these genes were determined, and the relevant phylogenetic trees are presented. Mutant HPr derivatives in which the regulatory serine was changed to either alanine or aspartate were constructed. The cloned galP gene was integrated into the chromosome of Bacillus subtilis, and synthesis of the mutant HPr proteins in this ...Continue Reading

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Citations

Jul 17, 2008·Nature Reviews. Microbiology·Boris Görke, Jörg Stülke
Apr 24, 2007·Applied and Environmental Microbiology·María Jesús YebraVicente Monedero
Mar 26, 2013·Applied and Environmental Microbiology·Zohra JamalMarguerite Dols-Lafargue
Mar 20, 2003·Journal of Bacteriology·Zhongge ZhangMilton H Saier
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherPieter W Postma
Dec 22, 2006·Journal of Molecular Microbiology and Biotechnology·Vicente MonederoJosef Deutscher
Nov 28, 2012·Biotechnology for Biofuels·Vasiliki TsakraklidesChristopher D Herring
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Apr 1, 2009·Microbiology·Jae-Han KimDavid A Mills
Nov 27, 2019·Cellular and Molecular Life Sciences : CMLS·Heejoon ParkRoss P Carlson

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