Genes involved in Listeria monocytogenes biofilm formation at a simulated food processing plant temperature of 15 °C

International Journal of Food Microbiology
Marta J PierceyLisbeth Truelstrup Hansen

Abstract

Listeria monocytogenes is a pathogenic foodborne bacterium whose persistence in food processing environments is in part attributed to its biofilm formation. Most biofilm studies have been carried out at 30-37 °C rather than at temperatures found in the food processing plants (i.e., 10-20 °C). The objective of the present study was to mine for novel genes that contribute to L. monocytogenes biofilm formation at 15 °C using the random insertional mutagenesis approach. A library of 11,024 L. monocytogenes 568 (serotype 1/2a) Himar1 insertional mutants was created. Mutants with reduced or enhanced biofilm formation at 15 °C were detected in microtiter plate assays with crystal violet and safranin staining. Fourteen mutants expressed enhanced biofilm phenotypes, and harbored transposon insertions in genes encoding cell wall biosynthesis, motility, metabolism, stress response, and cell surface associated proteins. Deficient mutants (n=5) contained interruptions in genes related to peptidoglycan, teichoic acid, or lipoproteins. Enhanced mutants produced significantly (p<0.05) higher cell densities in biofilm formed on stainless steel (SS) coupons at 15 °C (48 h) than deficient mutants, which were also more sensitive to benzalkonium ch...Continue Reading

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Citations

Sep 5, 2017·Pathogens·Angelo ColagiorgiAdriana Ianieri
May 1, 2018·NPJ Biofilms and Microbiomes·Harsh MathurR Paul Ross
Apr 16, 2020·Microorganisms·Jana PrzekwasEugenia Gospodarek-Komkowska
Nov 7, 2019·Journal of Food Science and Technology·Karine Lauer Cruz, Amanda de Souza da Motta
Sep 21, 2017·Medicines·Sabrina MacéH P Vasantha Rupasinghe
Jan 24, 2018·EFSA Journal·UNKNOWN EFSA Panel on Biological Hazards (BIOHAZ)Roland Lindqvist
Feb 16, 2021·Frontiers in Microbiology·Constanza MelianGraciela Margarita Vignolo
Jun 10, 2021·Microbial Biotechnology·Nika JanežJerica Sabotič

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