Genes involved in the methyl tert-butyl ether (MTBE) metabolic pathway of Mycobacterium austroafricanum IFP 2012

Microbiology
Nicolas Lopes FerreiraCharles W Greer

Abstract

Methyl tert-butyl ether (MTBE) is a persistent pollutant of surface and groundwater, and the reasons for its low biodegradability are poorly documented. Using one of the rare bacterial strains able to grow in the presence of MTBE, Mycobacterium austroafricanum IFP 2012, the protein profiles of crude extracts after growth in the presence of MTBE and glucose were compared by SDS-PAGE. Ten proteins with molecular masses of 67, 64, 63, 55, 50, 27, 24, 17, 14 and 11 kDa were induced after growth in the presence of MTBE. Partial amino acid sequences of N-terminal and internal peptide fragments of the 64 kDa protein were used to design degenerate oligonucleotide primers to amplify total DNA by PCR, yielding a DNA fragment that was used as a probe for cloning. A two-step cloning procedure was performed to obtain a 10 327 bp genomic DNA fragment containing seven ORFs, including a putative regulator, mpdR, and four genes, mpdC, orf1, mpdB and orf2, in the same cluster. The MpdB protein (64 kDa) was related to a flavoprotein of the glucose-methanol-choline oxidoreductase family, and the MpdC protein (55 kDa) showed a high similarity with NAD(P) aldehyde dehydrogenases. Heterologous expression of these gene products was performed in Mycoba...Continue Reading

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Citations

Jun 29, 2006·Applied Microbiology and Biotechnology·Nicolas Lopes FerreiraFrançoise Fayolle-Guichard
Mar 10, 2007·Applied Microbiology and Biotechnology·Nicolas Lopes FerreiraFrançoise Fayolle-Guichard
Jul 12, 2011·Applied and Environmental Microbiology·Franziska SchäferThore Rohwerder
Feb 24, 2015·Environmental Science and Pollution Research International·Shanshan LiWei Yan
Nov 3, 2012·Current Opinion in Biotechnology·Michael Hyman
Jan 13, 2015·Applied and Environmental Microbiology·Samanthi KottegodaMichael Hyman
Jan 23, 2021·Journal of Hazardous Materials·Jantiya Isanapong, Peerapong Pornwongthong

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