Genes of Different Catabolic Pathways Are Coordinately Regulated by Dal81 in Saccharomyces cerevisiae

Journal of Amino Acids
Marcos D PalavecinoMariana Bermúdez-Moretti

Abstract

Yeast can use a wide variety of nitrogen compounds. However, the ability to synthesize enzymes and permeases for catabolism of poor nitrogen sources is limited in the presence of a rich one. This general mechanism of transcriptional control is called nitrogen catabolite repression. Poor nitrogen sources, such as leucine, γ-aminobutyric acid (GABA), and allantoin, enable growth after the synthesis of pathway-specific catabolic enzymes and permeases. This synthesis occurs only under conditions of nitrogen limitation and in the presence of a pathway-specific signal. In this work we studied the temporal order in the induction of AGP1, BAP2, UGA4, and DAL7, genes that are involved in the catabolism and use of leucine, GABA, and allantoin, three poor nitrogen sources. We found that when these amino acids are available, cells will express AGP1 and BAP2 in the first place, then DAL7, and at last UGA4. Dal81, a general positive regulator of genes involved in nitrogen utilization related to the metabolisms of GABA, leucine, and allantoin, plays a central role in this coordinated regulation.

References

Aug 1, 1978·Journal of Bacteriology·R SumradaT Cooper
Jan 1, 1985·Advances in Microbial Physiology·J M WiameH N Arst
Jul 1, 1996·Nucleic Acids Research·U GüldenerJ H Hegemann
Feb 24, 2001·Molecular & General Genetics : MGG·P S NielsenH A Andersen
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 21, 2002·Methods in Enzymology·R Daniel Gietz, Robin A Woods
Jan 16, 2009·Biochemical Society Transactions·Per O Ljungdahl
Jun 29, 2011·Biochemical and Biophysical Research Communications·Sabrina B CardilloM Bermúdez Moretti

Citations

Mar 23, 2018·MSphere·Siobhan A TurnerGeraldine Butler
Feb 17, 2018·Scientific Reports·Ángel Vizoso-VázquezM Esperanza Cerdán
Oct 18, 2019·Microbiology and Molecular Biology Reviews : MMBR·Frans BianchiBert Poolman

Methods Mentioned

BETA
PCR
Immunoprecipitation
ChIP

Related Concepts

Allantoin
Enzyme Repression
Gamma-Aminobutyric Acid
Genes
Leucine
Metabolism
Nitrogen
Saccharomyces cerevisiae
Transcription, Genetic
Yeasts

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