Genes regulated by TorR, the trimethylamine oxide response regulator of Shewanella oneidensis

Journal of Bacteriology
Christophe BordiVincent Méjean

Abstract

The torECAD operon encoding the trimethylamine oxide (TMAO) respiratory system of Shewanella oneidensis is positively controlled by the TorS/TorR two-component system when TMAO is available. Activation of the tor operon occurs upon binding of the phosphorylated response regulator TorR to a single operator site containing the direct repeat nucleotide sequence TTCATAN4TTCATA. Here we show that the replacement of any nucleotide of one TTCATA hexamer prevented TorR binding in vitro, meaning that TorR specifically interacts with this DNA target. Identical direct repeat sequences were found in the promoter regions of torR and of the new gene torF (SO4694), and they allowed TorR binding to both promoters. Real-time PCR experiments revealed that torR is negatively autoregulated, whereas torF is strongly induced by TorR in response to TMAO. Transcription start site location and footprinting analysis indicate that the operator site at torR overlaps the promoter -10 box, whereas the operator site at torF is centered at -74 bp from the start site, in agreement with the opposite role of TorR in the regulation of the two genes. Since torF and torECAD are positively coregulated by TorR, we propose that the TorF protein plays a role related to...Continue Reading

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Citations

Sep 29, 2012·The Journal of Biological Chemistry·Kang-Mu LeeSang Sun Yoon
Mar 14, 2007·Nucleic Acids Research·Hongwei WuYing Xu
Jul 3, 2013·Applied and Environmental Microbiology·Dongru QiuJizhong Zhou
Apr 5, 2011·Journal of Bacteriology·Smadar ShulamiYuval Shoham
Nov 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lars Plate, Michael A Marletta
Apr 9, 2014·Environmental Microbiology·Anne-Xander van der StelMarc M S M Wösten
Apr 4, 2016·Extremophiles : Life Under Extreme Conditions·Sheng-Da ZhangLong-Fei Wu
Jun 18, 2005·Current Opinion in Biotechnology·James K Fredrickson, Margaret F Romine
Sep 14, 2019·Metallomics : Integrated Biometal Science·Arkadiusz ZupokSilke Leimkühler
Jun 5, 2020·The Journal of Biological Chemistry·Smadar ShulamiYuval Shoham
Jul 14, 2019·Applied and Environmental Microbiology·Olivier N LemaireChantal Iobbi-Nivol

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