Genes specifically modulated in sensitized skins allow the detection of sensitizers in a reconstructed human skin model. Development of the SENS-IS assay

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Françoise CottrezHervé Groux

Abstract

Analysis of genes modulated during the sensitization process either on mice (LLNA) or human (blisters) combined with data mining has allowed the definition of a comprehensive panel of sensitization biomarkers. This set of genes includes already identified markers such as the ARE family and others not yet associated with the sensitization process (the so-called SENS-IS gene subset). The expression of this set of genes has been measured on reconstituted human epidermis models (Episkin) exposed to various sensitizers and non-sensitizers. Fine analysis of their expression pattern indicates that it is the number of modulated genes rather than the intensity of up-regulation that correlates best with the sensitization potential of a chemical. Moreover, sensitizers that are weak inductors of ARE genes tend to be relevant modulators of the SENS-IS subset. By combining the expression data obtained with both gene subsets, it is now possible to identify a wide variety of sensitizers on a test system (in vitro reconstructed human epidermis) that is very similar to the in vivo situation and compatible with a large variety of test substance characteristics.

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Citations

Jan 23, 2016·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Françoise CottrezHervé Groux
Jul 22, 2015·Archives of Toxicology·Andreas Natsch, Roger Emter
Apr 12, 2016·Journal of the European Academy of Dermatology and Venereology : JEADV·U Blume-PeytaviR Dauskardt
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May 26, 2017·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Elodie ClouetPierre-Jacques Ferret

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