PMID: 11606805Oct 19, 2001Paper

Genes upregulated in lead-resistant glioma cells reveal possible targets for lead-induced developmental neurotoxicity

Toxicological Sciences : an Official Journal of the Society of Toxicology
P Li, T G Rossman

Abstract

Identifying genes upregulated in lead-resistant cells should give insight into lead toxicity and cellular protective mechanisms and may also result in identification of proteins that may be useful as biomarkers. Glial cells are thought to protect neurons against heavy metals. Rat glioma C6 cells share many properties of normal glial cells. To identify and analyze genes upregulated in a lead-resistant variant, PbR11, suppression subtractive hybridization (SSH) between mRNAs of wild-type and PbR11 cells was performed. Sequencing and database searches identified three genes, thrombospondin-1, heparin sulfate 6-sulfotransferase, and neuropilin-1, which play important roles in angiogenesis and axon growth during development. Two genes, HSP90 and UBA3, are involved in the ubiquitin-proteosome system. One gene was identified as that of a rat endogenous retrovirus and another, 2C9, is a transcript expressed in fos-transformed cells. PbR11 also overexpresses c-fos. Expression of these genes and effects of short-term lead exposure (24 h, up to 600 microM) on their expression in C6 cells was examined. The rat endogenous retrovirus and 2C9 are expressed only in PbR11 cells, and show no expression, either constitutive or lead-induced, in wi...Continue Reading

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Citations

Dec 4, 2003·Mutation Research·Ellen K Silbergeld
Sep 19, 2002·Journal of Neuroscience Research·Marie-Gabrielle ZurichFlorianne Monnet-Tschudi
Jun 28, 2018·Environmental Monitoring and Assessment·R Urrutia-GoyesN Ornelas-Soto
May 30, 2002·BMC Genomics·Wan JiKlaus Lindpaintner

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