Genetic aberrations in pediatric acute lymphoblastic leukemia by comparative genomic hybridization

Cancer Genetics and Cytogenetics
R KarhuJ Isola

Abstract

Classical cytogenetic analysis plays an important role in the diagnosis and classification of childhood acute lymphoblastic leukemia (ALL). However, poor in vitro growth of the malignant cells and suboptimal quality of metaphase spreads may sometimes cause false-negative findings (normal karyotype). We used comparative genomic hybridization (CGH) to study whether this new method is able to detect and characterize genetic aberrations not detected by karyotyping. CGH showed clonal genetic aberrations in 8 of 13 cases, most of which showed gains of several chromosomes, indicating hyperdiploidy. The sensitivity of CGH was sufficient to detect a small interstitial deletion of 6q. One karyotypically complex case was resolved by CGH showing a high-level amplification of DNA sequences originating from the 12p12-13. Interphase fluorescence in situ hybridization (FISH) analyses confirmed the CGH findings in 2 cases, validating the accuracy of CGH. In conclusion, CGH experiments established the known fact that hyperdiploidy is the most common finding in pediatric ALLs and that CGH may detect aberrations that are not seen in the G-banded karyotype. CGH was also able to further characterize genetic aberrations such as gene amplification, wh...Continue Reading

References

Dec 2, 1978·British Medical Journal·L M Secker-WalkerR M Hardisty
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·O P KallioniemiJ W Gray
Oct 21, 1991·Nucleic Acids Research·D K Lahiri, J I Nurnberger
Oct 1, 1990·Cancer Genetics and Cytogenetics·L M Secker-Walker
Nov 1, 1984·Cancer Genetics and Cytogenetics·D L WilliamsW Lemonds
Mar 1, 1995·Genes, Chromosomes & Cancer·M BentzP Lichter

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Citations

Jun 20, 2002·Cancer Genetics and Cytogenetics·Stéphanie StruskiPascale Cornillet-Lefebvre
Feb 13, 2001·Annales de génétique·G TachdjianF Viguié
May 26, 1999·Leukemia & Lymphoma·H Avet-Loiseau
Apr 28, 2004·Journal of Pediatric Hematology/oncology·Patricia Pérez-VeraRocío Ortíz
Oct 31, 2009·Journal of Pediatric Hematology/oncology·Louise Olde NordkampHenk van den Berg
Mar 10, 2001·Genes, Chromosomes & Cancer·H ElghezalS P Romana
Mar 29, 2000·Pediatric Hematology and Oncology·M RiceR L Stallings
Jul 9, 1999·The Journal of Pathology·L A James

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