PMID: 7022197Jul 1, 1981Paper

Genetic activity of actinomycin D in Saccharomyces cerevisiae but not in Escherichia coli

Mutation Research
E R NestmannD J Kowbel

Abstract

The potential of actinomycin D for induction of forward mutation (ADE-), reversion (TRP+), gene conversion, and mitotic recombination, was examined using haploid and diploid strains of yeast Saccharomyces cerevisiae. No increase in forward or reverse mutations or gene conversion was detected, but actinomycin D induced up to 13-fold increases in mitotic recombinants and a 2-fold increase in numbers of aberrant colonies, in a non-selective assay for genetic activity. Actinomycin D was non-mutagenic in a fluctuation test using Escherichia coli strain WP2 UvrA-. This furnishes an example of a mutagen which is negative in bacteria but has genetic activity in yeast, emphasizing the need for using a battery of microbial tests for determining the genetic activity of any given chemical.

References

Dec 1, 1975·Mutation Research·C R FisherS Snyder
Sep 1, 1978·Mutation Research·M A HannanT Brychcy
Feb 1, 1976·Mutation Research·M H GreenB A Bridges
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J McCannB N Ames
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·B N AmesF D Lee
Aug 30, 1974·Nature·M M Pater, S Mak
Aug 1, 1965·Experimental Cell Research·W Ostertag, W Kersten

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