Genetic Adaptation to Growth Under Laboratory Conditions in Escherichia coli and Salmonella enterica

Frontiers in Microbiology
Anna KnöppelDan I Andersson

Abstract

Experimental evolution under controlled laboratory conditions is becoming increasingly important to address various evolutionary questions, including, for example, the dynamics and mechanisms of genetic adaptation to different growth and stress conditions. In such experiments, mutations typically appear that increase the fitness under the conditions tested (medium adaptation), but that are not necessarily of interest for the specific research question. Here, we have identified mutations that appeared during serial passage of E. coli and S. enterica in four different and commonly used laboratory media and measured the relative competitive fitness and maximum growth rate of 111 genetically re-constituted strains, carrying different single and multiple mutations. Little overlap was found between the mutations that were selected in the two species and the different media, implying that adaptation occurs via different genetic pathways. Furthermore, we show that commonly occurring adaptive mutations can generate undesired genetic variation in a population and reduce the accuracy of competition experiments. However, by introducing media adaptation mutations with large effects into the parental strain that was used for the evolution ex...Continue Reading

References

Oct 1, 1979·Journal of Bacteriology·C WandersmanT Ferenci
Jan 1, 1972·Molecular & General Genetics : MGG·H Schmieger
Oct 1, 1983·Cell·S Lam, J R Roth
Sep 5, 1997·Science·F R BlattnerY Shao
Jun 7, 2001·Proceedings of the National Academy of Sciences of the United States of America·H M EllisD L Court
May 17, 2002·Current Biology : CB·Richard J EdwardsJohn F Y Brookfield
Aug 24, 2002·Journal of Bacteriology·Kazushi SuzukiTony Romeo
Apr 5, 2005·Journal of Bacteriology·Julianne H GroseJohn R Roth
Jun 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Robert WoodsRichard E Lenski
Aug 11, 2007·Science·Lília PerfeitoIsabel Gordo
Oct 2, 2007·Journal of Bacteriology·Guennadi SezonovRichard D'Ari
Feb 12, 2008·Current Protocols in Molecular Biology·Lynn C ThomasonDonald L Court
Jun 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Zachary D BlountRichard E Lenski
Jul 4, 2009·Journal of Agricultural and Food Chemistry·B Elliot HickmanYuan Yao
Nov 9, 2010·Proceedings of the National Academy of Sciences of the United States of America·Tom M ConradBernhard Ø Palsson
May 10, 2011·The Journal of Biological Chemistry·M Kenyon ApplebeeBernhard Ø Palsson
Jul 8, 2011·Molecular Systems Biology·Tom M ConradBernhard Ø Palsson
Aug 4, 2011·PLoS Pathogens·Erik GullbergDan I Andersson
Oct 25, 2011·Applied and Environmental Microbiology·Ian K BlabyEudes de Crécy
Jan 28, 2012·Science·Olivier TenaillonBrandon S Gaut
Feb 16, 2012·Molecular Ecology·Jeremy R DettmanRees Kassen
Jun 12, 2012·Genome Biology·Ram P MaharjanLei Wang
Jul 5, 2012·PLoS Genetics·Sanna KoskiniemiDan I Andersson
Dec 4, 2012·Applied and Environmental Microbiology·Pilar Eliana Puentes-TéllezJan Dirk van Elsas
Apr 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Sanna KoskiniemiChristopher S Hayes

❮ Previous
Next ❯

Citations

May 28, 2019·Molecular Biology and Evolution·Gerrit BrandisDiarmaid Hughes
Feb 12, 2020·Genome Biology and Evolution·Joshua L Cherry
Jul 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·César A LázaroCarlos A Conte-Junior
Nov 13, 2019·Proceedings of the National Academy of Sciences of the United States of America·Juhan KimShelley D Copley
Nov 29, 2020·Molecular Biology and Evolution·Katrin BartkeDiarmaid Hughes
Feb 2, 2021·PLoS Neglected Tropical Diseases·Jessica Z Kubicek-SutherlandNorman A Doggett
Nov 23, 2020·The Journal of Antimicrobial Chemotherapy·Gerrit BrandisDiarmaid Hughes
Oct 23, 2020·The Journal of Antimicrobial Chemotherapy·Lisa Praski AlzrigatDiarmaid Hughes
Mar 25, 2021·Molecular Biology and Evolution·Julia KloosPål J Johnsen
Apr 18, 2021·Current Opinion in Structural Biology·Shelley D Copley

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA433618
KM018298

Methods Mentioned

BETA
phosphotransferase
motility tests
PCR

Software Mentioned

CLCs Low Frequency Variant Detection
DIRex
CLC Genomics Workbench
CLC

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved