Genetic Analyses of Elys Mutations in Drosophila Show Maternal-Effect Lethality and Interactions with Nucleoporin Genes

G3 : Genes - Genomes - Genetics
Kazuyuki HiraiKyoichi Sawamura

Abstract

ELYS determines the subcellular localizations of Nucleoporins (Nups) during interphase and mitosis. We made loss-of-function mutations of Elys in Drosophila melanogaster and found that ELYS is dispensable for zygotic viability and male fertility but the maternal supply is necessary for embryonic development. Subsequent to fertilization, mitotic progression of the embryos produced by the mutant females is severely disrupted at the first cleavage division, accompanied by irregular behavior of mitotic centrosomes. The Nup160 introgression from D. simulans shows close resemblance to that of the Elys mutations, suggesting a common role for those proteins in the first cleavage division. Our genetic experiments indicated critical interactions between ELYS and three Nup107-160 subcomplex components; hemizygotes of either Nup37, Nup96 or Nup160 were lethal in the genetic background of the Elys mutation. Not only Nup96 and Nup160 but also Nup37 of D. simulans behave as recessive hybrid incompatibility genes with D. melanogaster An evolutionary analysis indicated positive natural selection in the ELYS-like domain of ELYS. Here we propose that genetic incompatibility between Elys and Nups may lead to reproductive isolation between D. melan...Continue Reading

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Citations

Nov 6, 2018·Canadian Journal of Physiology and Pharmacology·Claudia C PrestonRandolph S Faustino
Dec 17, 2020·Development·Valeria GuglielmiMaximiliano A D'Angelo

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Datasets Mentioned

BETA
GM22978
GM22979

Methods Mentioned

BETA
transgenic
PCR

Software Mentioned

Nups
BLAST
Elys
Adobe Photoshop
PAML
R
seqinR
ImageJ

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