Genetic Analysis Method for Staphylococcus chromogenes Associated with Goat Mastitis

Polish Journal of Microbiology
Rocío A Ruiz-RomeroEstela T Méndez-Olvera

Abstract

Mastitis in goats is mainly caused by coagulase-negative Staphylococcus (CNS). The identification methods for this group are based on evaluating the expression of phenotypic characteristics such as the ability to metabolize various substrates; however, this is disadvantageous as these methods are dependent on gene expression. In recent years, genotyping methods such as the Multiple Locus Variable-Number Tandem Repeat Analysis (MLVA) and gene identification have been useful for epidemiological study of several bacterial species. To develop a genotyping method, the genome sequence of Staphylococcus chromogenes MU970 was analysed. The analysis showed nine virulence genes described in Staphylococcus aureus. The MLVA was developed using four loci identified in the genome of S. chromogenes MU970. This genotyping method was examined in 23 strains of CNS isolated from goat mastitis. The rate of discrimination for MLVA was 0.8893, and the highest rates of discrimination per the index of Simpson and Hunter-Gaston were 0.926 and 0.968 for the locus 346_06, respectively. The virulence genes were present in all strains of S. chromogenes but not in other CNS. The genotyping method presented in this paper is a viable and easy method for typif...Continue Reading

References

Jun 10, 1998·Microbiology and Molecular Biology Reviews : MMBR·A van BelkumH Verbrugh
Dec 24, 1998·Nucleic Acids Research·G Benson
Aug 30, 2001·Journal of Clinical Microbiology·W J MasonM S Smeltzer
Oct 15, 2003·Veterinary Research·Dominique BergonierXavier Berthelot
Dec 8, 2004·Journal of Clinical Microbiology·Vincent RamisseGilles Vergnaud
Mar 15, 2006·Veterinary Microbiology·B-M ThorbergMarie-Louise Danielsson-Tham
Jan 16, 2007·International Journal of Systematic and Evolutionary Microbiology·Mohammad Monir ShahTakayuki Ezaki
Oct 10, 2008·Veterinary Microbiology·Ynte H SchukkenRuth N Zadoks
Oct 14, 2008·Veterinary Microbiology·Satu Pyörälä, Suvi Taponen
Oct 22, 2008·Veterinary Microbiology·Ruth N Zadoks, Jeffrey L Watts
Jun 13, 2009·Methods in Molecular Biology·Gilles Vergnaud, Christine Pourcel
Apr 29, 2011·Journal of Dairy Science·K SupréS De Vliegher
Oct 4, 2014·Clinical Microbiology Reviews·Karsten BeckerGeorg Peters
Dec 4, 2014·The Veterinary Journal·Wannes VanderhaeghenSarne De Vliegher
Jun 1, 2015·Microbial Pathogenesis·Sandra Renata Sampaio SalaberryNilson Roberti Benites
Apr 6, 2016·Gut Pathogens·Hongxia WangBiao Kan

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Datasets Mentioned

BETA
MU970

Methods Mentioned

BETA
PCR
genotyping
electrophoresis

Software Mentioned

- DICE )
Discriminator Power Calculator
BioNumerics
Tandem Repeats Finder
VNTR Diversity and Confidences Extraction ( V
DELL STATISTICA
DNAman
MLVA
BLAST Basic Local Alignment Search Tool

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