Genetic analysis of a Piezo-like protein suppressing systemic movement of plant viruses in Arabidopsis thaliana

Scientific Reports
Zhen ZhangXian-Bing Wang

Abstract

As obligate intracellular phytopathogens, plant viruses must take advantage of hosts plasmodesmata and phloem vasculature for their local and long-distance transports to establish systemic infection in plants. In contrast to well-studied virus local transports, molecular mechanisms and related host genes governing virus systemic trafficking are far from being understood. Here, we performed a forward genetic screening to identify Arabidopsis thaliana mutants with enhanced susceptibility to a 2b-deleted mutant of cucumber mosaic virus (CMV-2aT∆2b). We found that an uncharacterized Piezo protein (AtPiezo), an ortholog of animal Piezo proteins with mechanosensitive (MS) cation channel activities, was required for inhibiting systemic infection of CMV-2aT∆2b and turnip mosaic virus tagged a green fluorescent protein (GFP) (TuMV-GFP). AtPiezo is induced by virus infection, especially in the petioles of rosette leaves. Thus, we for the first time demonstrate the biological function of Piezo proteins in plants, which might represent a common antiviral strategy because many monocot and dicot plant species have a single Piezo ortholog.

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Citations

Jul 10, 2019·Molecular Plant Pathology·Hernan Garcia-Ruiz
Feb 17, 2021·Plant Signaling & Behavior·Xianming FangKai He
Sep 1, 2020·Journal of Experimental Botany·Long-Xiang ChaiXian-Bing Wang
Jan 21, 2021·International Journal of Molecular Sciences·Xianming FangKai He
Feb 10, 2021·NPJ Microgravity·Ken TakahashiKeiji Naruse
May 13, 2021·Proceedings of the National Academy of Sciences of the United States of America·Seyed A R MousaviArdem Patapoutian
Jul 31, 2021·Science·Ivan RadinElizabeth S Haswell
Sep 16, 2021·The Plant Cell·Jennette M CodjoeElizabeth S Haswell

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Methods Mentioned

BETA
PCR
transgenic

Software Mentioned

SPSS Statistics
AtPiezo
ImageJ
ClustalW
Integrative Genome Viewer
MEGA5
SAM

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