Genetic analysis of Ankamali pigs of India using microsatellite markers and their comparison with other domesticated Indian pig types

Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie
R BehlR K Vijh

Abstract

Ankamali pigs, the domesticated native pigs of Kerala province of India were genetically characterized using 23 FAO recommended microsatellite markers and were compared with other native Indian pig types and Large White pigs. Twenty-six blood samples were collected from genetically unrelated animals from their breeding tract and DNA was isolated by standard procedure of phenol/chloroform. The genomic DNA was amplified by polymerase chain reaction (PCR) at these 23 microsatellite loci, which were also used earlier to characterize Desi (North Indian) and Gahuri (North-East Indian) pigs, the other two native domesticated pig types of India. The PCR products were resolved by denaturing urea-polyacrylamide gel electrophoresis and alleles were visualized after silver nitrate staining. The data were analysed for allele size range, number of alleles, allelic frequencies, heterozygosity and polymorphism information content for each locus. The allele size range varied between 92-108 bp at locus S0026 and 280-296 bp at locus IGF-1. The total number of alleles varied between five (S0178 and S0386) and 11 (S0355). The mean observed and expected heterozygosities were found to be 0.74 +/- 0.09 and 0.83 +/- 0.03 respectively. In the neighbour-...Continue Reading

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Citations

Jun 4, 2011·Genetics and Molecular Biology·Anna Maria GuastellaSalvatore Bordonaro
Dec 24, 2015·Animal Biotechnology·Nihar Ranjan SahooMonalisa Sahoo
Sep 2, 2019·Mitochondrial DNA. Part B, Resources·Arun Kumar DeDebasis Bhattacharya

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