PMID: 2503195Jul 1, 1989Paper

Genetic analysis of DNA from single human oocytes: a model for preimplantation diagnosis of cystic fibrosis

BMJ : British Medical Journal
C CoutelleR Williamson

Abstract

Gene sequences in human oocytes were studied to investigate the possibility of diagnosing inherited or sporadic genetic disease before implantation after in vitro fertilisation. By specific amplification the possibility of analysing the DNA from single human oocytes for a specific gene was shown, and genotypes for markers closely linked to cystic fibrosis and Duchenne muscular dystrophy were determined. Single oocytes were used to approximate the total amount of DNA present in a single cell taken for biopsy from a 4-16 cell blastocyst. With a new technique for specific DNA amplification, the polymerase chain reaction, these data can be obtained within several hours of cell isolation. Extreme care must be taken to avoid any contamination of the sample with DNA from other sources. With this technique genotyping for single gene disorders is feasible with an accuracy and on a time scale that would allow implantation of the zygote after in vitro fertilisation without freezing.

References

Feb 18, 1989·Lancet·A H HandysideE G Tuddenham
Jun 18, 1988·Lancet·N LenchR Williamson
Sep 1, 1987·Human Genetics·D N Cooper, J Schmidtke
Sep 1, 1987·Baillière's Clinical Obstetrics and Gynaecology·R Penketh, A McLaren

❮ Previous
Next ❯

Citations

Aug 1, 1991·Journal of in Vitro Fertilization and Embryo Transfer : IVF·C M StromY Verlinsky
Feb 1, 1990·Journal of in Vitro Fertilization and Embryo Transfer : IVF·Y VerlinskyC Strom
Feb 1, 1994·Journal of Assisted Reproduction and Genetics·C M StromY Verlinsky
Sep 24, 1992·The New England Journal of Medicine·A H HandysideM R Hughes
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H LiN Arnheim
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A H LiuL J Wysocki
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·Z LinH Li
Jan 1, 1996·Journal of Inherited Metabolic Disease·W LissensI Liebaers
Mar 1, 1991·Baillière's Clinical Obstetrics and Gynaecology·J Yovich, A Lower
Mar 15, 2011·Reproductive Biomedicine Online·Anastasia A Theodosiou, Martin H Johnson
Jun 8, 2002·Expert Review of Molecular Diagnostics·Antony E Shrimpton
Jun 15, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·Alan H Handyside
Mar 1, 1995·Molecular Reproduction and Development·M B WheelerV L Jarrell
Dec 1, 1989·The Journal of Pathology·G Taylor, P Quirke
Jun 1, 1992·British Journal of Obstetrics and Gynaecology·N Morris, R Williamson
May 1, 1990·International Journal of STD & AIDS·P A Kitchin
Jan 1, 1992·Law, Medicine & Health Care : a Publication of the American Society of Law & Medicine·J C Fletcher, W F Anderson
Apr 1, 1996·Immunology and Cell Biology·A Saberivand, P M Outteridge
Jan 1, 1991·Comparative Immunology, Microbiology and Infectious Diseases·L Z Xu, D Larzul

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.