Genetic analysis of Giardia and Cryptosporidium from people in Northern Australia using PCR-based tools

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
Janine EbnerRobin B Gasser

Abstract

To date, there has been limited genetic study of the gastrointestinal pathogens Giardia and Cryptosporidium in northern parts of Australia. Here, PCR-based methods were used for the genetic characterization of Giardia and Cryptosporidium from 695 people with histories of gastrointestinal disorders from the tropical North of Australia. Genomic DNAs from fecal samples were subjected to PCR-based analyses of regions from the triose phosphate isomerase (tpi), small subunit (SSU) of the nuclear ribosomal RNA and/or the glycoprotein (gp60) genes. Giardia and Cryptosporidium were detected in 13 and four of the 695 samples, respectively. Giardia duodenalis assemblages A and B were found in 4 (31%) and 9 (69%) of the 13 samples in persons of <9 years of age. Cryptosporidium hominis (subgenotype IdA18), Cryptosporidium mink genotype (subgenotype IIA16R1) and C. felis were also identified in single patients of 11-21 years of age. Future studies might focus on a comparative study of these and other protists in rural communities in Northern Australia.

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Citations

Jan 19, 2016·Parasitology Research·Jessica Triviño-ValenciaJorge E Gomez-Marin
Sep 18, 2016·Environmental Science and Pollution Research International·Francisco Miroslav Ulloa-StanojlovićRonalda Silva de Araújo
Jul 28, 2016·Parasite Immunology·U RyanA Paparini
Nov 17, 2017·Clinical Microbiology Reviews·Lynne S GarciaGovinda Visvesvara
Sep 4, 2019·The Onderstepoort Journal of Veterinary Research·Regina D MiamboSamson Mukaratirwa
Oct 30, 2021·International Journal for Parasitology·Una M RyanLihua Xiao

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