PMID: 9546033Apr 18, 1998Paper

Genetic analysis of iron uptake in the yeast Saccharomyces cerevisiae

The Journal of Pediatrics
A Dancis

Abstract

We used the methods of yeast genetics to identify genes involved in acquisition of iron by eukaryotic cells. Mutants were identified with defects in cellular iron uptake. These were organized into an upstream group and a downstream group. The upstream group was involved in the delivery of copper to the multicopper oxidase FET3. Mutants of this group were characterized by defective iron uptake that could be corrected by exposure of the cells to large amounts of copper. The downstream group was more directly involved in iron uptake. Mutant phenotypes from these genes could not be corrected by copper exposure. Genes in the upstream group encoded the regulator of copper transport, MAC1, and two copper transporters, CTR1 and CCC2. Genes in the downstream group encoded the multicopper oxidase FET3 and its partner the iron permease FTR1. In addition, the downstream genes encoded the surface reductases FRE1 and FRE2 and the iron regulatory protein AFT1. The iron and copper uptake processes in yeast intersect because the FET3 gene encodes a multicopper oxidase that is required for iron transport. In human beings, an analogous function may be served by ceruloplasmin, a multicopper oxidase with a role in iron homeostasis.

References

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A DancisR D Klausner
Jan 1, 1991·Methods in Enzymology·F Sherman
May 1, 1990·Molecular and Cellular Biology·A DancisJ G Barriocanal
Dec 1, 1970·The Journal of Clinical Investigation·H P RoeserG E Cartwright
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·Z L HarrisJ D Gitlin
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·D S YuanR D Klausner
Jan 20, 1995·The Journal of Biological Chemistry·D M De SilvaJ Kaplan
Jun 14, 1996·The Journal of Biological Chemistry·K P ShatwellA W Segal

❮ Previous
Next ❯

Citations

Apr 17, 2009·Molecular Biology of the Cell·Laran T JensenValeria C Culotta
Aug 6, 2008·Annual Review of Phytopathology·Hubertus HaasB Gillian Turgeon
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·H H NguyenJ Stubbe
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·S R Davis-KaplanJ Kaplan
Feb 27, 2003·Molecular Microbiology·Daniel J Kosman
Feb 9, 2006·Fungal Genetics and Biology : FG & B·Yong-Sung ParkCheol-Won Yun
Jul 11, 2006·Biochimica Et Biophysica Acta·Valeria Cizewski CulottaThomas V O'Halloran
Oct 6, 2000·Annual Review of Microbiology·C Ratledge, L G Dover
Jun 22, 1999·The Journal of Physiology·A Rolfs, M A Hediger
Feb 4, 2009·The Journal of Cell Biology·Casey A Ydenberg, Mark D Rose
Oct 30, 2010·Molecular BioSystems·Praveen Kumar SharmaNilanjan Roy
Mar 27, 2012·FEMS Yeast Research·Citlali Fonseca-GarcíaJosé Ruiz-Herrera
Oct 3, 2012·Genetics·Haruo Saito, Francesc Posas
Jul 23, 2013·PloS One·Elahe RadmaneshfarMarco Thiel
Jul 9, 2013·Genetics·Lisa WasserstromJürgen Wendland
Feb 15, 2007·Molecular Systems Biology·Amnon AmirJoel Stavans
Dec 27, 2015·Medical Mycology·Mohammad T Albataineh, David Kadosh
Feb 4, 2010·The Biochemical Journal·Yoshifumi TakatsumeYoshiharu Inoue

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.