PMID: 1254639Apr 1, 1976

Genetic analysis of membrane differentiation in Paramecium. Freeze-fracture study of the trichocyst cycle in wild-type and mutant strains

The Journal of Cell Biology
J BeissonB H Satir

Abstract

Using a series of mutants of Paramecium tetraurelia, we demonstrate, for the first time, changes in the internal structure of the cell membrane, as revealed by freeze-fracture, that correspond to specific single gene mutations. On the plasma membrane of Paramecium circular arrays of particles mark the sites of attachment of the tips of the intracellular secretory organelles-trichocysts. In wild-type paramecia, where attached trichocysts can be expelled by exocytosis under various stimuli, the plasma membrane array is composed of a double outer ring of particles (300 nm in diameter) and inside the ring a central rosette (fusion rosette) of particles (76 nm in diameter). Mutant nd9, characterized by a thermosensitive ability to discharge trichocysts, shows the same organization in cells grown at the permissive temperature (18 degrees C), while in cells grown at the nonpermissive temperature (27 degrees C) the rosette is missing. In mutant tam 8, characterized by normal but unattached trichocysts, and in mutant tl, completely devoid of trichocysts, no rosette is formed and the outer rings always show a modified configuration called "parentheses", also found in wild-type and in nd9 (18 degrees C) cells. From this comparison between...Continue Reading

References

Jan 17, 1975·Nature·Q F AhkongJ A Lucy
Dec 1, 1974·The Journal of Cell Biology·A Elgsaeter, D Branton
Jan 1, 1974·Journal of Supramolecular Structure·B H Satir
Jan 1, 1973·Annual Review of Entomology·P Satir, N B Gilula
Jan 1, 1973·The Journal of Cell Biology·B H SatirP Satir
Nov 3, 1967·Science·D W Deamer, D Branton
May 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·D Branton
Oct 1, 1969·Experimental Cell Research·E SteersV T Marchesi

Citations

Jul 12, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·H Plattner
Jan 1, 1976·Journal of Supramolecular Structure·B H Satir
Apr 1, 1981·Journal of Neurocytology·B J McLaughlin, T S Reese
Nov 1, 1985·Mechanisms of Ageing and Development·K Aufderheide, M V Schneller
Jun 24, 2000·Biochimie·L VayssiéJean Cohen
Aug 1, 1996·Current Opinion in Cell Biology·J R Monck, J M Fernandez
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B H SatirE Orias
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B H SatirT J Murtaugh
Jun 1, 1977·The Journal of Cell Biology·S J Burwen, B H Satir
Dec 1, 1981·The Journal of Cell Biology·J J Rauh, D L Nelson
Apr 1, 1983·The Journal of Cell Biology·R S GarofaloB H Satir
Jul 1, 1983·The Journal of Cell Biology·D M Gilligan, B H Satir
Nov 17, 2010·Molecular Biology of the Cell·Randy Schekman
Aug 1, 1978·Biological Reviews of the Cambridge Philosophical Society·N de Terra
Feb 1, 1977·The Journal of protozoology·L E Roth, D J Pihlaja
Sep 1, 1991·The Journal of protozoology·J B Peterson
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·J C Hutton
Aug 1, 1992·Microscopy Research and Technique·L A Hufnagel
Apr 2, 1998·European Journal of Cell Biology·H Zhao, B H Satir
Jan 1, 1981·Progress in Histochemistry and Cytochemistry·J SchrévelM Monsigny
Jun 29, 1990·European Journal of Protistology·B Haacke-BellH Plattner
Oct 19, 1990·European Journal of Protistology·R Glas-AlbrechtH Plattner
Mar 10, 2007·Cellular Microbiology·Jean François Dubremetz
Feb 1, 1978·Journal of Ultrastructure Research·Jean-François Dubremetz, G Torpier
Jul 1, 1977·Journal of Ultrastructure Research·R Anderer, K Hausmann
Mar 1, 1984·Experimental Cell Research·H PlattnerR Stürzl
Jan 10, 1992·Biochimica Et Biophysica Acta·R Glas-AlbrechtH Plattner
Jul 1, 1989·The Journal of protozoology·B H Satir
Apr 1, 1988·Journal of Cellular Biochemistry·B H SatirT J Murtaugh
Jan 1, 1980·Annals of the New York Academy of Sciences·B H SatirN J Maihle
Jun 14, 1982·Biochimica Et Biophysica Acta·L M RiddleD L Nelson
Aug 14, 2003·Biochimica Et Biophysica Acta·H Plattner, R Kissmehl
Apr 2, 2004·Cell·Randy Schekman, Peter Novick
Feb 24, 2015·The Plant Journal : for Cell and Molecular Biology·Robert E Jinkerson, Martin C Jonikas
Apr 1, 1996·Histochemistry and Cell Biology·M KnochelR D Burgoyne
Jul 12, 2008·Nature Structural & Molecular Biology·William Wickner, Randy Schekman
May 29, 2000·European Journal of Biochemistry·A Galvani, L Sperling
Dec 27, 1977·Zeitschrift Für Parasitenkunde·E Porchet, G Torpier
Jan 27, 2021·Nature Microbiology·Eleonora AquiliniMaryse Lebrun
Mar 31, 2021·Nature Microbiology·Ke Hu

Related Concepts

Cell Differentiation Process
Plasma Membrane
Freeze Fracturing
Cistron
Organoids
Paramecium

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.