PMID: 1254639Apr 1, 1976

Genetic analysis of membrane differentiation in Paramecium. Freeze-fracture study of the trichocyst cycle in wild-type and mutant strains

The Journal of Cell Biology
J BeissonB H Satir


Using a series of mutants of Paramecium tetraurelia, we demonstrate, for the first time, changes in the internal structure of the cell membrane, as revealed by freeze-fracture, that correspond to specific single gene mutations. On the plasma membrane of Paramecium circular arrays of particles mark the sites of attachment of the tips of the intracellular secretory organelles-trichocysts. In wild-type paramecia, where attached trichocysts can be expelled by exocytosis under various stimuli, the plasma membrane array is composed of a double outer ring of particles (300 nm in diameter) and inside the ring a central rosette (fusion rosette) of particles (76 nm in diameter). Mutant nd9, characterized by a thermosensitive ability to discharge trichocysts, shows the same organization in cells grown at the permissive temperature (18 degrees C), while in cells grown at the nonpermissive temperature (27 degrees C) the rosette is missing. In mutant tam 8, characterized by normal but unattached trichocysts, and in mutant tl, completely devoid of trichocysts, no rosette is formed and the outer rings always show a modified configuration called "parentheses", also found in wild-type and in nd9 (18 degrees C) cells. From this comparison between...Continue Reading


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Jan 1, 1974·Journal of Supramolecular Structure·B H Satir
Jan 1, 1973·Annual Review of Entomology·P Satir, N B Gilula
Jan 1, 1973·The Journal of Cell Biology·B H SatirP Satir
Nov 3, 1967·Science·D W Deamer, D Branton
May 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·D Branton
Oct 1, 1969·Experimental Cell Research·E SteersV T Marchesi


Jul 12, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·H Plattner
Jan 1, 1976·Journal of Supramolecular Structure·B H Satir
Apr 1, 1981·Journal of Neurocytology·B J McLaughlin, T S Reese
Nov 1, 1985·Mechanisms of Ageing and Development·K Aufderheide, M V Schneller
Jun 24, 2000·Biochimie·L VayssiéJean Cohen
Aug 1, 1996·Current Opinion in Cell Biology·J R Monck, J M Fernandez
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B H SatirE Orias
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B H SatirT J Murtaugh
Jun 1, 1977·The Journal of Cell Biology·S J Burwen, B H Satir
Dec 1, 1981·The Journal of Cell Biology·J J Rauh, D L Nelson
Apr 1, 1983·The Journal of Cell Biology·R S GarofaloB H Satir
Jul 1, 1983·The Journal of Cell Biology·D M Gilligan, B H Satir
Nov 17, 2010·Molecular Biology of the Cell·Randy Schekman
Aug 1, 1978·Biological Reviews of the Cambridge Philosophical Society·N de Terra
Feb 1, 1977·The Journal of protozoology·L E Roth, D J Pihlaja
Sep 1, 1991·The Journal of protozoology·J B Peterson
Oct 6, 1997·Proceedings of the National Academy of Sciences of the United States of America·J C Hutton
Aug 1, 1992·Microscopy Research and Technique·L A Hufnagel
Apr 2, 1998·European Journal of Cell Biology·H Zhao, B H Satir
Jan 1, 1981·Progress in Histochemistry and Cytochemistry·J SchrévelM Monsigny
Jun 29, 1990·European Journal of Protistology·B Haacke-BellH Plattner
Oct 19, 1990·European Journal of Protistology·R Glas-AlbrechtH Plattner
Mar 10, 2007·Cellular Microbiology·Jean François Dubremetz
Feb 1, 1978·Journal of Ultrastructure Research·Jean-François Dubremetz, G Torpier
Jul 1, 1977·Journal of Ultrastructure Research·R Anderer, K Hausmann
Mar 1, 1984·Experimental Cell Research·H PlattnerR Stürzl
Jan 10, 1992·Biochimica Et Biophysica Acta·R Glas-AlbrechtH Plattner
Jul 1, 1989·The Journal of protozoology·B H Satir
Apr 1, 1988·Journal of Cellular Biochemistry·B H SatirT J Murtaugh
Jan 1, 1980·Annals of the New York Academy of Sciences·B H SatirN J Maihle
Jun 14, 1982·Biochimica Et Biophysica Acta·L M RiddleD L Nelson
Aug 14, 2003·Biochimica Et Biophysica Acta·H Plattner, R Kissmehl
Apr 2, 2004·Cell·Randy Schekman, Peter Novick
Feb 24, 2015·The Plant Journal : for Cell and Molecular Biology·Robert E Jinkerson, Martin C Jonikas
Apr 1, 1996·Histochemistry and Cell Biology·M KnochelR D Burgoyne
Jul 12, 2008·Nature Structural & Molecular Biology·William Wickner, Randy Schekman
May 29, 2000·European Journal of Biochemistry·A Galvani, L Sperling
Dec 27, 1977·Zeitschrift Für Parasitenkunde·E Porchet, G Torpier
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Mar 31, 2021·Nature Microbiology·Ke Hu

Related Concepts

Cell Differentiation Process
Plasma Membrane
Freeze Fracturing

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