PMID: 6412036Jan 1, 1983Paper

Genetic analysis of sacB, the structural gene of a secreted enzyme, levansucrase of Bacillus subtilis Marburg

Molecular & General Genetics : MGG
M SteinmetzP Gay

Abstract

The structural gene sacB encoding B. subtilis levansucrase, a secreted enzyme, expresses in E. coli. E. coli hosts of the sacB gene are poisoned by sucrose. This property allowed a powerful selection of mutants affected in the cloned gene. The plasmidic mutations were readily introduced in the B. subtilis chromosome. Using a collection of plasmids bearing various deletions extending in sacB we developed a technique of deletion mapping based on plasmid integration in the chromosome of B. subtilis. A generalization of this technique is discussed.

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Citations

Feb 18, 2005·Journal of Bacteriology·Thomas Bovbjerg RasmussenMichael Givskov
Sep 8, 2006·Applied and Environmental Microbiology·Daiva DailidieneDouglas E Berg
Mar 21, 2018·Journal of Bacteriology·Suneeta ChimalapatiKim Orth
May 26, 2020·BMC Microbiology·Francesca Romana CianfanelliDirk Bumann
Jun 4, 2013·Applied and Environmental Microbiology·Alexandra Faulds-Pain, Brendan W Wren
Apr 26, 2017·Microbial Cell Factories·Francesca ZerbiniGuido Grandi
Mar 30, 2018·Frontiers in Cellular and Infection Microbiology·Hana TlapákKlaus Heuner
Oct 23, 2015·Nature Protocols·Laura R HmeloJoe J Harrison
Oct 23, 2020·Proceedings of the National Academy of Sciences of the United States of America·Robin A SorgJan-Willem Veening
Oct 29, 1998·FEMS Immunology and Medical Microbiology·S ContiL Polonelli
Dec 5, 2002·Tuberculosis·S-H LeeJ N Maslow
Nov 1, 1996·Applied and Environmental Microbiology·M G Bramucci, V Nagarajan
Aug 26, 1998·Infection and Immunity·J M ReyratR Rappuoli
May 1, 1989·Plasmid·K Weston-Hafer, D E Berg
Jan 1, 1986·Annales De L'Institut Pasteur. Microbiology·M Steinmetz, S Aymerich

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