Genetic analysis of signal transduction through light-induced protein phosphorylation in Neurospora crassa perithecia

Molecular & General Genetics : MGG
K Oda, Kohji Hasunuma

Abstract

We have previously demonstrated that blue light induces the phosphorylation of a 15-kDa protein in crude membrane fractions of Neurospora crassa mycelia. Here we report the isolation and characterization of a mutant (psp; phosphorylation of small proteins that is completely defective for phosphorylation of that protein, as assayed in both crude membrane and soluble fractions. This mutation defines a unique locus that maps to linkage group VR between al-3 and his-6. To elucidate the photobiological significance of the phosphorylation of the protein, we analyzed known photobiological phenomena and discovered that the positioning of beaks on the perithecia, defined as perithecial polarity, was light-dependent in the wild type. In the psp mutant, beaks were phototropic as in the wild type, but their position was random. In a wc-l mutant, however, beaks were positioned at random and were not phototropic. Thus light-induced perithecial polarity and phototropism of perithecial beaks are controlled differently. A psp; wc-l double mutant showed the same phenotype as that of wc-l with respect to these two photomorphogenetic characters. These results indicate that the wc-l gene is epistatic to psp in the light-signal transduction pathway ...Continue Reading

Citations

Jan 30, 2003·Fungal Genetics and Biology : FG & B·María D Quiles-RosilloVictoriano Garre
Dec 3, 1999·European Journal of Biochemistry·Y OguraK Hasunuma
Nov 3, 2011·Journal of Signal Transduction·Tatiana A BelozerskayaYulia I Deryabina
Aug 24, 2013·Antioxidants & Redox Signaling·Norbert Gyöngyösi, Krisztina Káldi
Nov 20, 2003·The Journal of Biological Chemistry·Yusuke Yoshida, Kohji Hasunuma
Oct 29, 2003·Plant & Cell Physiology·Yosuke FukamatsuKohji Hasunuma
Nov 22, 2016·BioMed Research International·Li Hua TangYing Wang
Jul 1, 2019·Biochemical and Biophysical Research Communications·Asmita D PawarYogendra Sharma
Jan 15, 2017·Microbiology Spectrum·Reinhard FischerLuis M Corrochano

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