Genetic analysis of the CDI pathway from Burkholderia pseudomallei 1026b

PloS One
Sanna KoskiniemiDavid A Low

Abstract

Contact-dependent growth inhibition (CDI) is a mode of inter-bacterial competition mediated by the CdiB/CdiA family of two-partner secretion systems. CdiA binds to receptors on susceptible target bacteria, then delivers a toxin domain derived from its C-terminus. Studies with Escherichia coli suggest the existence of multiple CDI growth-inhibition pathways, whereby different systems exploit distinct target-cell proteins to deliver and activate toxins. Here, we explore the CDI pathway in Burkholderia using the CDIIIBp1026b system encoded on chromosome II of Burkholderia pseudomallei 1026b as a model. We took a genetic approach and selected Burkholderia thailandensis E264 mutants that are resistant to growth inhibition by CDIIIBp1026b. We identified mutations in three genes, BTH_I0359, BTH_II0599, and BTH_I0986, each of which confers resistance to CDIIIBp1026b. BTH_I0359 encodes a small peptide of unknown function, whereas BTH_II0599 encodes a predicted inner membrane transport protein of the major facilitator superfamily. The inner membrane localization of BTH_II0599 suggests that it may facilitate translocation of CdiA-CTIIBp1026b toxin from the periplasm into the cytoplasm of target cells. BTH_I0986 encodes a putative transgly...Continue Reading

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Citations

Aug 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Julia L E WillettChristopher S Hayes
Sep 22, 2015·Journal of Molecular Biology·Julia L E WillettChristopher S Hayes
Jul 3, 2015·Molecular Microbiology·Zachary C RuheChristopher S Hayes
Sep 22, 2016·BMC Bioinformatics·Vesna MemiševićJaques Reifman
Nov 1, 2017·Emerging Topics in Life Sciences·Allison M JonesChristopher S Hayes
Oct 31, 2020·International Journal of Molecular Sciences·Larisa N IkryannikovaAndrey A Zamyatnin
Oct 28, 2017·Current Opinion in Microbiology·Erin C Garcia
Jun 11, 2019·Journal of Molecular Biology·Nicholas L BartelliFrederick W Dahlquist

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Methods Mentioned

BETA
PCR
flow cytometry
electrophoresis

Software Mentioned

Jalview

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