Genetic analysis of the glutamate permease in Escherichia coli K-12.

Journal of Bacteriology
M Marcus, Y S Halpern

Abstract

The glutamate permeation system in Escherichia coli K-12 consists of three genes: gltC, gltS, and gltR. The genes gltC and gltS are very closely linked, and are located between the pyrE and tna loci, in the following order: tna, gltC, gltS, pyrE; gltR is located near the metA gene. The three glt genes constitute a regulatory system in which gltR is the regulator gene responsible for the formation of repressor, gltS is the structural gene of the glutamate permease, and gltC is most probably the operator locus. The synthesis of glutamate permease is partially repressed in wild-type K-12 strains, resulting in the inability of these strains to utilize glutamate as the sole source of carbon. Derepression due to mutation at the gltC locus enables growth on glutamate as a carbon source both at 30 C and at 42 C. Temperature-sensitive gltR mutants capable of utilizing glutamate for growth at 42 C but not at 30 C were found to be derepressed for glutamate permease when grown at 42 C and partially repressed (wild-type phenotype) upon growth at 30 C. These mutants produce an altered thermolabile repressor which can be inactivated by mild heat treatment (10 min at 44 C) in the absence of growth.

References

Dec 1, 1967·Bacteriological Reviews·A L Taylor, C D Trotter
Nov 1, 1965·Journal of Bacteriology·Y S Halpern, M Lupo
Mar 1, 1967·Journal of Bacteriology·Y S Halpern, A Even-Shoshan
Apr 1, 1967·Journal of Bacteriology·M Marcus, Y S Halpern
Jul 1, 1966·Genetics·M DemerecP E Hartman
Feb 1, 1957·Journal of General Microbiology·W HAYES
Sep 1, 1960·Journal of Bacteriology·Y S HALPERN, H E UMBARGER
Jun 1, 1961·Journal of Molecular Biology·F JACOB, J MONOD
Feb 26, 1960·Science·L GORINI, H KAUFMAN
Jan 1, 1964·Archives of Biochemistry and Biophysics·G F AMES
Feb 1, 1965·Journal of Bacteriology·T K GARTNER, M RILEY
Mar 1, 1965·Biochemistry·D KESSEL, M LUBIN
Jun 1, 1965·Journal of Molecular Biology·J R SADLER, A NOVICK
Mar 1, 1962·Journal of Bacteriology·M LUBIN

❮ Previous
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Citations

Jul 11, 2014·Antonie van Leeuwenhoek·Pilar Eliana Puentes-Téllez, Jan Dirk van Elsas
Mar 1, 1991·Molecular & General Genetics : MGG·M KalmanM Cashel
Dec 22, 2009·Molecular BioSystems·Matthew S LuchanskyJonathan D Dattelbaum
Mar 1, 1976·Bacteriological Reviews·B J BachmannA L Taylor
Oct 1, 1970·Journal of Bacteriology·K D Brown
Dec 1, 1981·Journal of Bacteriology·A A Stark, S A Yankofsky
May 1, 1982·Journal of Bacteriology·S A RosenfeldJ E Brenchley
Aug 1, 1970·Journal of Bacteriology·M Lupo, Y S Halpern
Jun 1, 1970·Bacteriological Reviews·A L Taylor
Jan 1, 1987·Comparative Biochemistry and Physiology. A, Comparative Physiology·N IvanovE Toncheva
Oct 1, 1969·Journal of Bacteriology·L Frank, I Hopkins
Apr 1, 1978·Journal of Bacteriology·S J Hochhauser, B Weiss
Jan 1, 1971·Journal of Bacteriology·K Krajewska-GrynkiewiczT Klopotowski
Mar 1, 1969·Journal of Bacteriology·M Marcus, Y S Halpern
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn
Nov 5, 1971·Biochemical and Biophysical Research Communications·H Barash, Y S Halpern
Dec 1, 1993·Microbiological Reviews·M Riley

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