Genetic analysis of the herpes simplex virus type 1 UL20 protein domains involved in cytoplasmic virion envelopment and virus-induced cell fusion

Journal of Virology
Jeffrey M MelanconKonstantin G Kousoulas

Abstract

The herpes simplex virus type 1 UL20 protein (UL20p) is an important determinant for cytoplasmic virion morphogenesis and virus-induced cell fusion. To delineate the functional domains of the UL20 protein, we generated a panel of single and multiple (cluster) alanine substitutions as well as UL20p carboxyl-terminal truncations. The UL20 mutant genes could be broadly categorized into four main groups: Group I UL20 mutant genes complemented for both virus production and virus-induced cell fusion; Group II UL20 mutant genes did not complement for either virus-induced cell fusion or infectious virus production; Group III UL20 mutant genes complemented for virus-induced cell fusion to variable extents but exhibited substantially decreased ability to complement UL20-null infectious virus production; Group IV mutant genes complemented for infectious virus production but had variable effects on virus-induced cell fusion; this group included two mutants that efficiently complemented for gBsyn3, but not for gKsyn1, virus-induced cell fusion. In addition, certain recombinant viruses with mutations in either the amino or carboxyl termini of UL20p produced partially syncytial plaques on Vero cells in the absence of any other virally encoded...Continue Reading

References

Apr 1, 1991·The Journal of General Virology·C A MacLeanD J McGeoch
Dec 1, 1982·The Journal of General Virology·P G SandersA J Davison
Sep 1, 1995·Journal of Virology·L Hutchinson, D C Johnson
Jan 1, 1993·Annual Review of Cell Biology·I S TrowbridgeC R Hopkins
Jan 1, 1996·Methods in Molecular Biology·A AiyarJ Leis
Jan 1, 1996·Annual Review of Cell and Developmental Biology·I Mellman
Jul 1, 1997·Journal of Virology·W FuchsT C Mettenleiter
Aug 1, 1997·Current Opinion in Cell Biology·T KirchhausenH Riezman
Mar 12, 1999·Journal of Virology·R S Tirabassi, L W Enquist
May 9, 2000·Journal of Virology·P DietzT C Mettenleiter
Jun 26, 2001·Traffic·M J TomishimaL W Enquist
Dec 12, 2001·Journal of Virology·David C Johnson, Mary T Huber
Jan 19, 2002·Journal of Virology·Thomas C Mettenleiter
Jun 14, 2003·Journal of Virology·Susan L BjerkeRichard J Roller

❮ Previous
Next ❯

Citations

Dec 15, 2012·The Journal of General Virology·Gen TakahashiYoshiaki Yura
Jun 10, 2008·Immunity·Miguel RellosoD Branch Moody
Oct 3, 2006·Virology·Susan L HallThomas C Heineman
Dec 17, 2014·Journal of Virology·Imane El Kasmi, Roger Lippé
Dec 19, 2018·Current Clinical Microbiology Reports·Paul J F RiderKonstantin G Kousoulas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.