Genetic Analysis of the Listeria Pathogenicity Island 1 of Listeria monocytogenes 1/2a and 4b Isolates

Current Microbiology
Agni HadjiloukaEleftherios H Drosinos

Abstract

The aim of the present study was to apply descriptive, phylogenetic, recombination, and selection analyses on alignments of the Listeria Pathogenicity Island 1 (LIPI-1) of 1/2a and 4b Listeria monocytogenes isolates of different origin in order to gain insights into the evolution of this virulence gene cluster. For that purpose, a total of 19 L. monocytogenes isolates (9 meat isolates, serotype 1/2a; 5 meat isolates, serotype 4b; 5 strawberry isolates, serotype 4b) that have been previously separated at strain level were subjected to sequencing of their LIPI-1. Descriptive analysis revealed extensive nucleotide diversity mostly in the intragenic regions. The actA gene of 1/2a and 4b meat isolates and the hly gene of the 4b strawberry isolates exhibited the higher diversity; limited diversity was observed in prfA and plcA genes of the 4b isolates and mpl gene of the 1/2a isolates. Phylogenetic analysis of the complete island resulted in two major clusters that were consistent with serotype assignment of the isolates. Moreover, effective discrimination between serotypes was obtained by plcA, plcB, mpl, actA and the intergenic regions plcA-prfA and plcA-hly. In all cases but plcB and plcA-prfA 4b isolates were also differentiated ...Continue Reading

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Dec 5, 2014·Journal of Food Protection·Agni HadjiloukaEleftherios H Drosinos

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Citations

Mar 19, 2020·Molecular Microbiology·Fernando BaqueroTeresa M Coque
Dec 19, 2019·Frontiers in Microbiology·Bart FerwerdaDiederik van de Beek

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Methods Mentioned

BETA
PCR

Software Mentioned

GeneStudio Professional Edition
MEGA
DnaSP
SplitsTree
hly

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