PMID: 2500416Jul 1, 1989Paper

Genetic analysis of the promoter region of the Bacillus subtilis alpha-amylase gene

Journal of Bacteriology
M J Weickert, G H Chambliss

Abstract

The amyR2 allele of the Bacillus subtilis alpha-amylase cis-regulatory region enhances production of amylase and transcription of amyE, the structural gene, by two- to threefold over amyR1. The amylase gene bearing each of these alleles was cloned on plasmids of about 10 to 15 copies per chromosome. Transcription of the cloned amylase gene by each amyR allele was activated at the end of exponential growth and was subject to catabolite repression by glucose. The amount of amylase produced was roughly proportional to the copy number of the plasmid, and cells containing the amyR2-bearing plasmid, pAR2, produced two- to threefold more amylase than cells with the amyR1 plasmid, pAMY10. Deletion of DNA 5' to the alpha-amylase promoter, including deletion of the A + T-rich inverted repeat found in amyR1 and amyR2, had no effect on expression or transcription of alpha-amylase. Deletion of DNA 3' to the amyR1 promoter did not impair temporal activation of chloramphenicol acetyltransferase in amyR1-cat-86 transcriptional fusions, but catabolite repression was abolished. When an 8-base-pair linker was inserted in pAMY10 at the same site from which the 3' deletion was made, amylase expression doubled and was repressed less by glucose. Both...Continue Reading

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Citations

Apr 15, 1997·FEMS Microbiology Letters·T NishidaT Uozumi
Oct 27, 2004·FEMS Microbiology Letters·Christopher M BrowngardtRobert A Burne
Jul 1, 1990·Journal of Bacteriology·B Oskouian, G C Stewart

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