Genetic analysis of the Replication Protein A large subunit family in Arabidopsis reveals unique and overlapping roles in DNA repair, meiosis and DNA replication

Nucleic Acids Research
Behailu B AkliluKevin M Culligan

Abstract

Replication Protein A (RPA) is a heterotrimeric protein complex that binds single-stranded DNA. In plants, multiple genes encode the three RPA subunits (RPA1, RPA2 and RPA3), including five RPA1-like genes in Arabidopsis. Phylogenetic analysis suggests two distinct groups composed of RPA1A, RPA1C, RPA1E (ACE group) and RPA1B, RPA1D (BD group). ACE-group members are transcriptionally induced by ionizing radiation, while BD-group members show higher basal transcription and are not induced by ionizing radiation. Analysis of rpa1 T-DNA insertion mutants demonstrates that although each mutant line is likely null, all mutant lines are viable and display normal vegetative growth. The rpa1c and rpa1e single mutants however display hypersensitivity to ionizing radiation, and combination of rpa1c and rpa1e results in additive hypersensitivity to a variety of DNA damaging agents. Combination of the partially sterile rpa1a with rpa1c results in complete sterility, incomplete synapsis and meiotic chromosome fragmentation, suggesting an early role for RPA1C in promoting homologous recombination. Combination of either rpa1c and/or rpa1e with atr revealed additive hypersensitivity phenotypes consistent with each functioning in unique repair pa...Continue Reading

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Citations

Feb 10, 2016·Frontiers in Plant Science·Behailu B Aklilu, Kevin M Culligan
Dec 30, 2015·Journal of Experimental Botany·Chaolong WangJianmin Wan
Nov 2, 2015·Chromosoma·Jonathan RibeiroEmmanuelle Martini
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May 20, 2016·Nucleic Acids Research·José Antonio Pedroza-GarciaCécile Raynaud
Mar 1, 2018·Annual Review of Plant Biology·Yingxiang Wang, Gregory P Copenhaver
Aug 23, 2019·Proceedings of the National Academy of Sciences of the United States of America·Kezhen YangJie Le
May 3, 2019·BMC Evolutionary Biology·Xueyuan Jiang, Raquel Assis
Jun 6, 2020·Frontiers in Plant Science·Jiayu LuJianmin Wan
Oct 2, 2019·International Journal of Molecular Sciences·Jose-Antonio Pedroza-GarciaCécile Raynaud
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Nov 12, 2015·Frontiers in Plant Science·Vasilissa Manova, Damian Gruszka
Apr 29, 2021·Journal of Experimental Botany·Wenxuan ZouJie Zhao
May 29, 2021·Planta·Supriyo ChowdhurySupriya Chakraborty
Nov 7, 2021·The Plant Journal : for Cell and Molecular Biology·José Antonio Pedroza-GarciaLieven De Veylder

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Methods Mentioned

BETA
PCR
confocal microscopy

Software Mentioned

ClustalW
ImageJ
MEGA
Qcapture

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