Genetic analysis of the UL 15 gene locus for the putative terminase of herpes simplex virus type 1

Virology
D Yu, S K Weller

Abstract

The herpes simplex virus (HSV-1) UL15 gene encodes one of the six viral gene products required for viral DNA cleavage and packaging. UL15 is a spliced gene and encodes two separately translated proteins, UL15 and UL15.5. Sequence analysis reveals that UL15 shares homology with gp 17, the large catalytic subunit of the bacteriophage T4 terminase, a protein which cleaves the polymeric T4 DNA into monomers. Both proteins contain a putative ATP binding motif known as the Walker A and B boxes. In this report, immunofluorescence was used to show that UL15 localizes to the nucleus in the absence of any other viral proteins; this indicates that UL15 contains its own nuclear localization signal. In addition, we found that UL15 colocalizes with replication compartments at early times (6 h postinfection). Since, at this time, preformed capsids as well as other cleavage and packaging proteins are also recruited to replication compartments, it seems likely that cleavage and packaging occurs in the same compartments in which DNA synthesis occurs. Also in this report, we have investigated UL15.5, the N-terminally truncated gene product of the UL15 open reading frame (ORF). The start codon has been mapped to Met443 within the UL15 ORF. Further...Continue Reading

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Citations

Jun 26, 2009·Journal of Virology·Frauke BeilsteinNigel D Stow
Dec 14, 2012·Journal of Virology·Sophia PavlovaHenri-Jacques Delecluse
Sep 2, 2011·Journal of Virology·Mindy LeelawongGregory A Smith
Feb 14, 2006·Journal of Virology·Brian R BowmanFlorante A Quiocho
Oct 22, 2008·The Journal of General Virology·Jian Ben Wang, Michael A McVoy
Jun 7, 2002·Molecular Cell·Tonny de BeerCarlos Enrique Catalano
Jan 8, 2008·Virology·Devon A Gregory, Steven L Bachenheimer
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Mar 25, 2018·Reviews in Medical Virology·Ellyn M Kornfeind, Robert J Visalli
Dec 18, 2001·The Journal of Biological Chemistry·Jae-Seon Hwang, Elke Bogner

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