Genetic analysis of transvection effects involving cis-regulatory elements of the Drosophila Ultrabithorax gene.

Genetics
J L MicolA García-Bellido

Abstract

The Ultrabithorax (Ubx) gene of Drosophila melanogaster contains two functionally distinguishable regions: the protein-coding Ubx transcription unit and, upstream of it, the transcribed but nonprotein-coding bxd region. Numerous recessive, partial loss-of-function mutations which appear to be regulatory mutations map within the bxd region and within the introns of the Ubx transcription unit. In addition, mutations within the Ubx unit exons are known and most of these behave as null alleles. Ubx1 is one such allele. We have confirmed that, although the Ubx1 allele does not produce detectable Ubx proteins (UBX), it does retain other genetic functions detectable by their effects on the expression of a paired, homologous Ubx allele, i.e., by transvection. We have extended previous analyses made by E. B. Lewis by mapping the critical elements of the Ubx gene which participate in transvection effects. Our results show that the Ubx1 allele retains wild-type functions whose effectiveness can be reduced (1) by additional cis mutations in the bxd region or in introns of the Ubx transcription unit, as well as (2) by rearrangements disturbing pairing between homologous Ubx genes. Our results suggest that those remnant functions in Ubx1 are...Continue Reading

Citations

Mar 20, 1996·Molecular & General Genetics : MGG·A Martínez-LabordaJ L Micol
Jul 12, 1996·Cell·R Aramayo, R L Metzenberg
Aug 1, 1992·Molecular & General Genetics : MGG·J E Castelli-GairA García-Bellido

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