PMID: 9539764May 16, 1998Paper

Genetic analysis using genomic representations

Proceedings of the National Academy of Sciences of the United States of America
Robert LucitoMichael Wigler

Abstract

Analysis of the genetic changes in human tumors is often problematical because of the presence of normal stroma and the limited availability of pure tumor DNA. However, large amounts of highly reproducible "representations" of tumor and normal genomes can be made by PCR from nanogram amounts of restriction endonuclease cleaved DNA that has been ligated to oligonucleotide adaptors. We show here that representations are useful for many types of genetic analyses, including measuring relative gene copy number, loss of heterozygosity, and comparative genomic hybridization. Representations may be prepared even from sorted nuclei from fixed and archived tumor biopsies.

References

May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L C ChenE Liu
Mar 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J Ben-EzraA Wu
Feb 1, 1991·American Journal of Clinical Pathology·C E GreerM M Manos
Jul 1, 1991·Computer Applications in the Biosciences : CABIOS·T J Gleeson, R Staden
Sep 1, 1995·European Journal of Biochemistry·V Joulin, H Richard-Foy
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·J JuR A Mathies
Feb 12, 1993·Science·N LisitsynM Wigler
Apr 1, 1996·Human Pathology·M A HermsenJ J Walboomers
May 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Schena
Jan 1, 1996·Clinical Oncology : a Journal of the Royal College of Radiologists·C C Vernon
Aug 1, 1996·Cancer Genetics and Cytogenetics·N LemieuxB Dutrillaux
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·M SchenaR W Davis
Oct 1, 1996·Genome Research·U E GibsonP M Williams
Jul 18, 2000·Journal of Agricultural and Food Chemistry·M L FauconnierM Paquot

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Citations

Apr 10, 2010·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Cornelia LangeHeinz Himmelbauer
Nov 25, 2004·Progress in Biophysics and Molecular Biology·Simon HughesJeremy Squire
Feb 1, 2003·Biochemical and Biophysical Research Communications·Kazuhiko AoyagiHiroki Sasaki
Feb 1, 2005·The Journal of Molecular Diagnostics : JMD·Sandy DevriesFrederic Waldman
Aug 6, 2002·Nature Biotechnology·G Mike MakrigiorgosBrendan D Price
Apr 20, 2005·Nature Genetics·Kevin L GundersonMark S Chee
May 3, 2008·Nature Protocols·Ludmila KousoulidouPhilippos C Patsalis
Jul 19, 2006·Nature Reviews. Genetics·Jian-Bing FanKevin L Gunderson
Nov 23, 2006·European Journal of Human Genetics : EJHG·Philippos C PatsalisAnts Kurg
Feb 14, 2006·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Joseph WestBud Mishra
Jul 4, 2001·Nucleic Acids Research·Y ZhangG M Makrigiorgos
Sep 18, 2007·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Evi MichelsFrank Speleman
Feb 6, 2004·Genome Research·Graham R BignellRichard Wooster
Nov 3, 2004·Genome Research·Gang WangG Mike Makrigiorgos
Apr 5, 2000·Molecular Pathology : MP·M M WeissP J van Diest
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·C A KleinG Riethmüller
Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·J StolarovV Mittal
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Masaaki HamaguchiMichael H Wigler
Nov 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Raoul-Sam DaruwalaBud Mishra
Jan 27, 2006·The Journal of Molecular Diagnostics : JMD·Jin LiG Mike Makrigiorgos
Jun 21, 2006·Human Mutation·Lovisa Lovmar, Ann-Christine Syvänen
Sep 22, 2005·Molecular and Biochemical Parasitology·Céline Karine CarretAlasdair Ivens
Mar 18, 2015·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Jie ZhangEnze Liu
Sep 2, 1999·Nature Genetics·J R PollackP O Brown
Sep 10, 2003·Nature Biotechnology·Giulia C KennedyKeith W Jones
Mar 19, 2008·Nature Reviews. Genetics·David GreshamDavid Botstein
Dec 2, 2005·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Grace NgNicholas Coleman
May 29, 2004·Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B·Dongxin LiuRam H Datar

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