Genetic Ancestry Analysis Reveals Misclassification of Commonly Used Cancer Cell Lines

Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology
Stanley HookerRick A Kittles

Abstract

Given the scarcity of cell lines from underrepresented populations, it is imperative that genetic ancestry for these cell lines is characterized. Consequences of cell line mischaracterization include squandered resources and publication retractions. We calculated genetic ancestry proportions for 15 cell lines to assess the accuracy of previous race/ethnicity classification and determine previously unknown estimates. DNA was extracted from cell lines and genotyped for ancestry informative markers representing West African (WA), Native American (NA), and European (EUR) ancestry. Of the cell lines tested, all previously classified as White/Caucasian were accurately described with mean EUR ancestry proportions of 97%. Cell lines previously classified as Black/African American were not always accurately described. For instance, the 22Rv1 prostate cancer cell line was recently found to carry mixed genetic ancestry using a much smaller panel of markers. However, our more comprehensive analysis determined the 22Rv1 cell line carries 99% EUR ancestry. Most notably, the E006AA-hT prostate cancer cell line, classified as African American, was found to carry 92% EUR ancestry. We also determined the MDA-MB-468 breast cancer cell line carrie...Continue Reading

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Citations

Sep 26, 2020·Journal of Cancer Education : the Official Journal of the American Association for Cancer Education·Altovise T EwingLori H Erby
Nov 14, 2019·Health Equity·Nancy BreenPhilip E Bourne
Oct 31, 2020·Cell·Leanne Woods-Burnham
Jul 18, 2021·Critical Reviews in Oncology/hematology·Julieta Abraham-MirandaKosj Yamoah

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