Genetic and alkaloid analysis of Papaver species and their F1 hybrid by RAPD, HPLC and ELISA

Forensic Science International : Synergy
Y ShoyamaK Nishi

Abstract

Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum Lindle and P. pseudo-orientale Medw. Analysis of random-amplified polymorphic DNA (RAPD) using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.

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Citations

Mar 26, 2004·Forensic Science International : Synergy·Keizo HosokawaAtsuyuki Hishida
Sep 2, 2005·The Journal of Heredity·A K ShasanyS P S Khanuja
Jul 13, 2007·Phytochemical Analysis : PCA·Jue ZhouRui Nan
Feb 9, 2018·Scientific Reports·Michael A MarcianoKevin S Sweder
Jun 29, 1999·Analytical Chemistry·T A BrettellR Saferstein

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