Genetic and biochemical characterization of periplasmic-leaky mutants of Escherichia coli K-12.

Journal of Bacteriology
J C Lazzaroni, R Portalier

Abstract

Periplasmic-leaky mutants of Escherichia coli K-12 were isolated after nitrosoguanidine-induced mutagenesis. They released periplasmic enzymes into the extracellular medium. Excretion of alkaline phosphatase, which started immediately in the early exponential phase of growth, could reach up to 90% of the total enzyme production in the stationary phase. Leaky mutants were sensitive to ethylenediaminetetraacetic acid, cholic acid, and the antibiotics rifampin, chloramphenicol, mitomycin C, and ampicillin. Furthermore, they were resistant to colicin E1 and partially resistant to phage TuLa. Their genetic characterization showed that the lky mutations mapped between the suc and gal markers, near or in the tolPAB locus. A biochemical analysis of cell envelope components showed that periplasmic-leaky mutants contained reduced amounts of major outer membrane protein OmpF and increased amounts of a 16,000-dalton outer membrane protein.

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Citations

Sep 1, 1989·Molecular & General Genetics : MGG·J C LazzaroniR Portalier
Aug 1, 1986·Molecular & General Genetics : MGG·J C LazzaroniR C Portalier
Feb 29, 2000·Journal of Bacteriology·A J Heilpern, M K Waldor
Jan 1, 1985·Molecular & General Genetics : MGG·J C Lazzaroni, R C Portalier
Mar 10, 2007·Microbiology and Molecular Biology Reviews : MMBR·Eric CascalesDanièle Cavard
Aug 26, 2009·Molecular Microbiology·C BaudF Jacob-Dubuisson
Apr 16, 2002·Molecular Microbiology·D Scott MerrellAndrew Camilli
Jan 1, 1983·Molecular & General Genetics : MGG·M Heyde, R Portalier
Mar 23, 2011·Journal of Bacteriology·Lois M BantaPeter J Christie
May 31, 2020·FEMS Microbiology Reviews·Joanna SzczepaniakColin Kleanthous
Aug 18, 2017·Molecular Microbiology·Rahul ShrivastavaShu-Sin Chng
Apr 14, 2018·Engineering in Life Sciences·Gabriele R M Kleiner-GroteKarl Friehs
Nov 13, 2020·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Hideyuki SuzukiHidehiko Kumagai

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