Genetic and Chemical-Genetic Interactions Map Biogenesis and Permeability Determinants of the Outer Membrane of Escherichia coli.

MBio
Kristina KlobucarEric D Brown

Abstract

Gram-negative bacteria are intrinsically resistant to many antibiotics due to their outer membrane barrier. Although the outer membrane has been studied for decades, there is much to uncover about the biology and permeability of this complex structure. Investigating synthetic genetic interactions can reveal a great deal of information about genetic function and pathway interconnectivity. Here, we performed synthetic genetic arrays (SGAs) in Escherichia coli by crossing a subset of gene deletion strains implicated in outer membrane permeability with nonessential gene and small RNA (sRNA) deletion collections. Some 155,400 double-deletion strains were grown on rich microbiological medium with and without subinhibitory concentrations of two antibiotics excluded by the outer membrane, vancomycin and rifampin, to probe both genetic interactions and permeability. The genetic interactions of interest were synthetic sick or lethal (SSL) gene deletions that were detrimental to the cell in combination but had a negligible impact on viability individually. On average, there were ∼30, ∼36, and ∼40 SSL interactions per gene under no-drug, rifampin, and vancomycin conditions, respectively; however, many of these involved frequent interactors...Continue Reading

References

Dec 1, 1977·The Journal of Antibiotics·K S Rosenthal, D R Storm
Oct 1, 1976·Journal of Bacteriology·G M Decad, H Nikaido
Sep 1, 1992·Microbiological Reviews·M Vaara
May 1, 1992·Molecular Microbiology·P Plésiat, H Nikaido
Feb 1, 1989·Antimicrobial Agents and Chemotherapy·D M ShlaesR Williamson
Sep 1, 1988·FEMS Microbiology Reviews·H M KuhnH Mayer
May 1, 1985·Journal of Bacteriology·P D RickD Bitter-Suermann
Nov 22, 1965·Biochemical and Biophysical Research Communications·L Leive
Feb 1, 1981·Journal of Bacteriology·L BeutinN Willetts
Nov 1, 1993·Antimicrobial Agents and Chemotherapy·M Vaara
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Jun 5, 2002·Annual Review of Biochemistry·Christian R H Raetz, Chris Whitfield
Oct 3, 2003·The Journal of Biological Chemistry·Chiou-Yan Lai, John E Cronan
Nov 18, 2003·Annual Review of Genetics·Justin Courcelle, Philip C Hanawalt
Dec 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Hiroshi Nikaido
Dec 17, 2005·Nature Reviews. Microbiology·Natividad RuizThomas J Silhavy
Apr 25, 2006·Biostatistics·W Evan JohnsonAriel Rabinovic
Jun 6, 2006·Gene·Simanti DattaDonald L Court
Jun 14, 2006·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Masanari KitagawaHirotada Mori
Sep 7, 2006·Journal of Endotoxin Research·M Stephen TrentJessica V Hankins
Aug 5, 2008·Nature Methods·Gareth ButlandAndrew Emili
Dec 23, 2008·Biochimica Et Biophysica Acta·Anne H Delcour
Jan 23, 2009·Nature Methods·Athanasios TypasCarol A Gross
Mar 4, 2010·Microbiology and Molecular Biology Reviews : MMBR·Terri N Ellis, Meta J Kuehn
Mar 20, 2010·The EMBO Journal·Tsuyoshi UeharaThomas G Bernhardt
May 11, 2010·Cold Spring Harbor Perspectives in Biology·Thomas J SilhavySuzanne Walker
Jun 1, 2010·Cell·Pedro BeltraoNevan J Krogan
Jul 27, 2011·PloS One·Fran SupekTomislav Šmuc
Aug 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Zhizhong YaoNatividad Ruiz
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri

❮ Previous
Next ❯

Methods Mentioned

BETA
SGA
PCR
PCRs

Software Mentioned

NetworkAnalyzer
OMI Explorer
R
REVIGO
ImageJ
sva package
SNE
Outer Membrane Interaction ( OMI ) Explorer
OSIRIS Datawarrior
LOESS

Related Concepts

Related Feeds

Bacterial Protein Structures (ASM)

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.