Genetic and Chromosomal Differentiation of Rhamdia quelen (Siluriformes, Heptapteridae) Revealed by Repetitive Molecular Markers and DNA Barcoding

Zebrafish
Mariana Campaner UssoAna Lúcia Dias

Abstract

Rhamdia quelen, a species of Heptapteridae, is considered to be a complex because of taxonomic and phylogenetic inconsistencies. Determining the physical location of repetitive DNA sequences on the chromosomes and the DNA barcode might increase our understanding of these inconsistencies within different groups of fish. To this end, we analyzed R. quelen populations from two river basins in Brazil, Paraguay and Parana, using DNA barcoding and different chromosomal markers, including U2 snDNA, which has never been analyzed for any Rhamdia species. Cytochrome c oxidase I gene sequence analysis revealed a significant differentiation among populations from the Miranda and Quexada rivers, with genetic distances compatible to those found among different species in neotropical fishes. Our results, in general, revealed a conservative chromosomal evolution in R. quelen and a differential distribution of some markers, such as 5S rDNA and U2 snDNA, in different populations. We suggest that R. quelen must undergo a major revision in its morphological, genetic, and cytogenetic molecular and taxonomic structure to elucidate possible operational taxonomic units.

References

May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D PinkelJ W Gray
Jan 1, 1980·Cytogenetics and Cell Genetics·M R Lee, F F Elder
Jan 1, 1995·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·S SalvadoriO Zuffardi
Oct 9, 1999·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·C Martins, P M Galetti
Nov 25, 2004·Heredity·K F KavalcoO Moreira-Filho
Oct 11, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Robert D WardPaul D N Hebert
Oct 11, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mehrdad HajibabaeiPaul D N Hebert
Oct 26, 2005·Current Opinion in Chemical Biology·Saba Valadkhan
Jan 5, 2008·Genetica·Marcelo Ricardo VicariLuiz Antonio Carlos Bertollo
Oct 1, 2010·Genetics and Molecular Research : GMR·J R TsudaA L Dias
Jun 28, 2011·Mitochondrial DNA·Daniel C de CarvalhoRobert Hanner
Mar 19, 2013·BMC Genetics·Luiz H G PereiraClaudio Oliveira
Sep 26, 2013·Cytogenetic and Genome Research·T S SczepanskiR F Artoni
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar

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Methods Mentioned

BETA
PCR

Software Mentioned

MEGA
Electropherogram Quality Analysis
BLAST

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