Genetic and enzymatic experiments relating to the tertiary structure of beta-galactosidase.

Journal of Bacteriology
J Langridge

Abstract

Fifty-six amber mutations of the beta-galactosidase gene of Escherichia coli were suppressed by crossing into a stock containing the supD suppressor gene. The resultant enzymes, differing only in the position of the inserted serine, were tested for stability at 57 C. Most of the suppressed enzymes were either as stable to heat as the normal enzyme or very unstable. Tests of enzymes produced by the action of other suppressors showed that the degree of stability was characteristic of a particular position in the polypeptide chain of the amino acid substitution and independent of the amino acid inserted. The mutations were placed in linear order in the gene by deletion mapping and three-point linkage tests. The consequent order of the serine substitutions disclosed an alternating pattern of stable and unstable regions over the amino-terminal two-thirds of the enzyme; the carboxy-terminal third of the enzyme was generally unstable. Considerations of coding relations and enzyme structure suggested that serine and glutamine suppression usually result in a change in the hydrophilic nature of the side chains on the outside of the enzyme molecule. It was shown that the potentially unstable regions of the enzyme are probably not indicati...Continue Reading

References

Feb 1, 1965·Mutation Research·A EisenstarkR Van Sickle
Aug 1, 1966·Journal of Molecular Biology·S BrennerA O Stretton
May 13, 1967·Nature·B W MatthewsD M Blow
Jan 1, 1961·Advances in Protein Chemistry·P URNES, P DOTY
Mar 1, 1964·Journal of Molecular Biology·J R BECKWITH
Aug 1, 1964·Journal of Molecular Biology·D R DAVIES

❮ Previous
Next ❯

Citations

Jun 14, 1978·Molecular & General Genetics : MGG·P J Davies, J M Parry
Oct 18, 1976·Molecular & General Genetics : MGG·T W Seale
Jun 19, 1975·Molecular & General Genetics : MGG·J Langridge
Dec 1, 1984·Biochemical Genetics·J Van Loon, R M Weinshilboum
Sep 1, 1984·Journal of Neurochemistry·W F YoungR M Weinshilboum
Jul 7, 1972·Nature·R Holliday, G M Tarrant
Jan 1, 1968·Molecular & General Genetics : MGG·J Langridge
Jun 7, 1974·Nature·P Talmud, D Lewis
Jan 1, 1978·CRC Critical Reviews in Microbiology·R E Amelunxen, A L Murdock
Jan 1, 1969·Molecular & General Genetics : MGG·J Langridge, J H Campbell
Nov 28, 1970·Nature·C M Lewis, R Holliday
Feb 1, 1974·Genetical Research·P J Talmud, D Lewis
Apr 1, 1985·Journal of Neurochemistry·W F YoungR M Weinshilboum
Jan 1, 1987·Biotechnology & Genetic Engineering Reviews·P L BergquistW H Morgan
Mar 1, 1988·Cellular and Molecular Neurobiology·R Weinshilboum
Jun 1, 1994·Chemico-biological Interactions·R Weinshilboum, I Aksoy
Jan 1, 1989·Pharmacology & Therapeutics·R Weinshilboum
Jan 1, 1990·Pharmacology & Therapeutics·R Weinshilboum
Aug 26, 1975·Biochimica Et Biophysica Acta·G P Moore, D T Sullivan
Sep 25, 1974·Journal of Molecular Biology·P JargielloP Hoffee
Aug 1, 1988·Clinical Biochemistry·R Weinshilboum
Apr 7, 1984·Journal of Theoretical Biology·G R Lambert

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.