Genetic and epidemiological analysis of norovirus from children with gastroenteritis in Botswana, 2013-2015

BMC Infectious Diseases
Kgomotso MakhaolaLemme P Kebaabetswe

Abstract

Norovirus is a leading cause of viral gastroenteritis worldwide with a peak of disease seen in children. The epidemiological analysis regarding the virus strains in Africa is limited. The first report of norovirus in Botswana was in 2010 and currently, the prevalence and circulating genotypes of norovirus are unknown, as the country has no systems to report the norovirus cases. This study investigated the prevalence, patterns and molecular characteristics of norovirus infections among children ≤5 years of age admitted with acute gastroenteritis at four hospitals in Botswana. A total of 484 faecal samples were collected from children who were admitted with acute gastroenteritis during the rotavirus vaccine impact survey between July 2013 and December 2015. Norovirus was detected using real-time RT-PCR. Positive samples were genotyped using conventional RT-PCR followed by partial sequencing of the capsid and RdRp genes. Norovirus strains were determined by nucleotide sequence analysis using the online Norovirus Genotyping Tool Version 1.0, and confirmed using maximum likelihood tree construction as implemented in MEGA 6.0. The prevalence of norovirus was 9.3% (95% CI 6.7-11.9). The genotype diversity was dominated by the GII.4 st...Continue Reading

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Datasets Mentioned

BETA
MF817977
MF818009
MF920449.1
MF920478.1

Methods Mentioned

BETA
nucleic acid extraction
PCR
Genotyping

Software Mentioned

Sequencher®
Molecular Evolutionary Genetic Analysis ( MEGA )

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