Genetic and functional analyses of the øX174 DNA binding protein: the effects of substitutions for amino acid residues that spatially organize the two DNA binding domains

Virology
Susan HafensteinBentley A Fane

Abstract

The øX174 DNA binding protein contains two DNA binding domains, containing a series of DNA binding basic amino acids, separated by a proline-rich linker region. Within each DNA binding domain, there is a conserved glycine residue. Glycine and proline residues were mutated and the effects on virion structure were examined. Substitutions for glycine residues yield particles with similar properties to previously characterized mutants with substitutions for DNA binding residues. Both sets of mutations share a common extragenic second-site suppressor, suggesting that the defects caused by the mutant proteins are mechanistically similar. Hence, glycine residues may optimize DNA-protein contacts. The defects conferred by substitutions for proline residues appear to be fundamentally different. The properties of the mutant particles along with the atomic structure of the virion suggest that the proline residues may act to guide the packaged DNA to the adjacent fivefold related asymmetric unit, thus preventing a chaotic packaging arrangement.

References

Jan 1, 1979·Journal of Bacteriology·F WinstonJ H Miller
Nov 16, 1978·Nature·G N GodsonJ C Fiddes
Oct 25, 1978·Journal of Molecular Biology·F SangerM Smith
Oct 1, 1983·Journal of Biomolecular Structure & Dynamics·M G RossmannH S Savithri
Sep 1, 1980·Cell·W C Earnshaw, S R Casjens
Apr 15, 1994·Journal of Molecular Biology·R McKennaM G Rossmann
Jan 14, 1994·Journal of Molecular Biology·J P WeryJ E Johnson
Jan 20, 1997·Virology·B Jennings, B A Fane
Sep 26, 1997·Nature·T DoklandM G Rossmann
Feb 5, 1999·Journal of Molecular Biology·A D BurchB A Fane
Feb 27, 1999·Nature Structural Biology·B BothnerG Siuzdak
May 18, 1999·Journal of Molecular Biology·T DoklandM G Rossmann
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·M A KrolP Ahlquist
Jul 20, 2001·Journal of Virology·C FliggeM Sapp
Oct 3, 2002·The Journal of Biological Chemistry·Andrea T Da PoianJerson L Silva
Dec 11, 2002·Journal of Molecular Biology·Ricardo A BernalMichael G Rossmann

❮ Previous
Next ❯

Citations

Mar 30, 2004·Journal of Molecular Biology·Ricardo A BernalMichael G Rossmann
Feb 14, 2016·Virology·Sarah M Doore, Bentley A Fane
May 31, 2015·Molecular Biology and Evolution·Sarah M Doore, Bentley A Fane
Sep 25, 2015·Journal of Virology·Jian GuanSusan Hafenstein
Jul 1, 2016·Journal of Virology·Aaron P Roznowski, Bentley A Fane
Jun 26, 2020·Viruses·Aaron P RoznowskiBentley A Fane
Nov 18, 2016·Virology·Sarah M DooreBentley A Fane

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Related Papers

Journal of Molecular Biology
B W Matthews
Proceedings of the National Academy of Sciences of the United States of America
J K LewisG Siuzdak
Journal of Molecular Biology
Ricardo A BernalM G Rossmann
Acta Crystallographica. Section D, Biological Crystallography
Collaborative Computational Project, Number 4
© 2021 Meta ULC. All rights reserved