Genetic and functional analysis of HIV type 1 nef gene derived from long-term nonprogressor children: association of attenuated variants with slow progression to pediatric AIDS

AIDS Research and Human Retroviruses
Guillermo CorróLuisa Sen

Abstract

Among persons infected by HIV-1, the rate of progression to AIDS is multifactorial being affected by host and viral factors, including the HIV-encoded negative factor (Nef). Our aim was to define whether variations in the nef gene as well as its functions may be associated with slower HIV disease course in infected children. The proviral HIV-1 nef gene was cloned, sequenced, and compared in children with contrasting disease course: 10 long-term nonprogressors (LTNP) and six rapid progressor (RP). The CD4 and MHC-I down-modulation ability of nef alleles derived from LTNP and RP children was analyzed. We observed that only one of our 10 LTNP had a protective genetic background, and out of them, 40% had defective nef genes, carrying substitutions at the (AWLEAQ(56-61)) and the (Rxx(22-24)) domains, and that those alleles were unable of down-regulate CD4 and MHC-I. The emergence or presence of Nef L58V substitution was associated with viral attenuation, indicated by a reduction in HIV viral loads, a persistent preservation of CD4(+) T cell counts, and lack of AIDS-related symptoms. Our results demonstrate that HIV-1 perinatally infected children carrying functionally defective nef HIV-1 strains have prolonged asymptomatic phases wi...Continue Reading

References

Dec 28, 1989·The New England Journal of Medicine·G B ScottW P Parks
Jan 26, 1995·The New England Journal of Medicine·F KirchhoffR C Desrosiers
Dec 16, 2000·AIDS Research and Human Retroviruses·R GeffinA S Baur
Jul 21, 2001·EMBO Reports·M GeyerB M Peterlin
Sep 22, 2001·BMJ : British Medical Journal·H Saloojee, A Violari
Dec 26, 2001·Bioinformatics·S KumarM Nei
May 8, 2003·Journal of Biosciences·Alison L GreenwayMichael Lidman
Mar 27, 2004·Current HIV Research·Cleo G Anastassopoulou, Leondios G Kostrikis
Sep 29, 2004·Journal of Virology·Ming-Bo HuangVincent C Bond
Jan 11, 2005·Current HIV Research·Ajith M JosephDebashis Mitra
Jan 20, 2006·HIV Medicine·R ChakrabortyS L Rowland-Jones
Mar 3, 2007·AIDS Research and Human Retroviruses·Polly R WalkerLynn Morris
Apr 15, 2008·Microbes and Infection·Jonathan M Carlson, Zabrina L Brumme
Sep 24, 2008·Retrovirology·John L Foster, J Victor Garcia

❮ Previous
Next ❯

Citations

Jan 8, 2013·Retrovirology·Philip MwimanziTakamasa Ueno
Sep 18, 2013·Retrovirology·Jaclyn K MannZabrina L Brumme
Feb 25, 2014·Journal of the International AIDS Society·Guillermo CorróLuisa Sen
Sep 24, 2014·Case Reports in Infectious Diseases·B H RimawiT C Dixon
Sep 1, 2015·Journal of Immunological Methods·Gursev AnmoleMark A Brockman
Jan 6, 2018·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Robert PaulStephen Kerr

❮ Previous
Next ❯

Datasets Mentioned

BETA
AF385936
JF283601
JF283778

Methods Mentioned

BETA
flow cytometry
PCR
myristoylation

Software Mentioned

LIFEMATCH
BioEdit
bootscan
SimPlot
Clustal X
FACSDiva
MEGA
GraphPad Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.