Genetic and functional characterization of clonally derived adult human brown adipocytes.

Nature Medicine
Kosaku ShinodaShingo Kajimura

Abstract

Brown adipose tissue (BAT) acts in mammals as a natural defense system against hypothermia, and its activation to a state of increased energy expenditure is believed to protect against the development of obesity. Even though the existence of BAT in adult humans has been widely appreciated, its cellular origin and molecular identity remain elusive largely because of high cellular heterogeneity within various adipose tissue depots. To understand the nature of adult human brown adipocytes at single cell resolution, we isolated clonally derived adipocytes from stromal vascular fractions of adult human BAT from two individuals and globally analyzed their molecular signatures. We used RNA sequencing followed by unbiased genome-wide expression analyses and found that a population of uncoupling protein 1 (UCP1)-positive human adipocytes possessed molecular signatures resembling those of a recruitable form of thermogenic adipocytes (that is, beige adipocytes). In addition, we identified molecular markers that were highly enriched in UCP1-positive human adipocytes, a set that included potassium channel K3 (KCNK3) and mitochondrial tumor suppressor 1 (MTUS1). Further, we functionally characterized these two markers using a loss-of-functio...Continue Reading

References

Mar 5, 2003·BioTechniques·A I SaeedJ Quackenbush
May 5, 2004·The Journal of Biological Chemistry·Sandrine NouetClara Nahmias
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·James A TimmonsBarbara Cannon
May 3, 2007·American Journal of Physiology. Endocrinology and Metabolism·Jan NedergaardBarbara Cannon
Aug 23, 2008·Nature·Patrick SealeBruce M Spiegelman
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Apr 10, 2009·The New England Journal of Medicine·Wouter D van Marken LichtenbeltG J Jaap Teule
Apr 10, 2009·The New England Journal of Medicine·Aaron M CypessC Ronald Kahn
Apr 10, 2009·The New England Journal of Medicine·Kirsi A VirtanenPirjo Nuutila
Jan 7, 2010·BMC Bioinformatics·Ben C Stöver, Kai F Müller
Dec 3, 2010·International Journal of Molecular Medicine·Per-Arne SvenssonLena M S Carlsson
Aug 11, 2011·American Journal of Physiology. Endocrinology and Metabolism·Tomas B WaldénJan Nedergaard
Nov 21, 2012·PloS One·Louis Z SharpShingo Kajimura
Dec 12, 2012·Nature Biotechnology·Cole TrapnellLior Pachter
Mar 19, 2013·Cell Metabolism·Sona RajakumariPatrick Seale
Apr 16, 2013·Cell Metabolism·Francesc Villarroya, Antonio Vidal-Puig
Apr 23, 2013·Nature Medicine·Martin E LidellSven Enerbäck
Jun 6, 2013·International Journal of Obesity : Journal of the International Association for the Study of Obesity·P LeeF S Celi
Jul 23, 2013·The Journal of Clinical Investigation·Takeshi YoneshiroMasayuki Saito
Jul 23, 2013·The Journal of Clinical Investigation·Anouk A J J van der LansWouter D van Marken Lichtenbelt
Oct 9, 2013·Nature Medicine·Matthew Harms, Patrick Seale
Nov 6, 2013·Annual Review of Physiology·Shingo Kajimura, Masayuki Saito
Apr 9, 2014·Cell Metabolism·Jonathan Z LongBruce M Spiegelman
Sep 25, 2014·Diabetic Medicine : a Journal of the British Diabetic Association·E SøndergaardN Jessen

❮ Previous
Next ❯

Citations

Feb 16, 2016·Cell Metabolism·Katrin J SvenssonBruce M Spiegelman
Feb 15, 2016·Trends in Cell Biology·Joan Sanchez-GurmachesDavid A Guertin
Jan 28, 2016·Obesity·Agnieszka Chabowska-Kita, Leslie P Kozak
Aug 6, 2015·Cell Metabolism·Labros S SidossisDavid N Herndon
Jun 9, 2015·Diabetes·Paul Cohen, Bruce M Spiegelman
Jan 20, 2016·Cell Biochemistry and Function·Ryosuke KidaMasayuki Funaba
Dec 17, 2015·Nature Communications·Changtao JiangFrank J Gonzalez
Oct 27, 2015·Frontiers in Endocrinology·Yannick JeansonLouis Casteilla
Mar 12, 2016·Nature Reviews. Drug Discovery·Antonio GiordanoSaverio Cinti
Apr 30, 2015·International Journal of Obesity : Journal of the International Association for the Study of Obesity·M ChondronikolaL S Sidossis
Oct 9, 2015·Cell Metabolism·Shingo KajimuraPatrick Seale
Oct 7, 2015·Frontiers in Endocrinology·Xiaoyong Yang, Hai-Bin Ruan
Sep 18, 2015·Frontiers in Endocrinology·Wei Zhang, Sheng Bi
Sep 1, 2015·Frontiers in Endocrinology·Jun Hong ParkSean Bong Lee
May 6, 2016·Frontiers in Endocrinology·Sung Sik ChoeJae Bum Kim
Aug 2, 2015·Biotechnology Advances·Andrea M UnserYubing Xie
May 7, 2016·International Journal of Obesity Supplements·S Kajimura
Apr 9, 2015·Trends in Genetics : TIG·Jun WuJoseph R McDermott
May 1, 2016·Cell and Tissue Research·Outi HuttalaT Ylikomi
Jul 8, 2016·Adipocyte·Claude ForestPhilippe Noirez
Jun 3, 2016·Nature Reviews. Molecular Cell Biology·Takeshi InagakiShingo Kajimura
Jun 14, 2016·Cell Metabolism·Mohammed K HankirWiebke K Fenske
Aug 9, 2016·Molecular and Cellular Endocrinology·Cristina ContrerasMiguel López
Aug 11, 2016·Cell Metabolism·Craig PorterLabros S Sidossis
Aug 16, 2016·Endocrinology and Metabolism Clinics of North America·Anny Mulya, John P Kirwan
Sep 10, 2016·Biochemical and Biophysical Research Communications·Osamu HashimotoMasayuki Funaba
Oct 21, 2015·Trends in Endocrinology and Metabolism : TEM·Sander KooijmanPatrick C N Rensen
Aug 30, 2016·Cell Metabolism·Svetlana Altshuler-KeylinShingo Kajimura
Oct 5, 2016·Best Practice & Research. Clinical Endocrinology & Metabolism·Florian W Kiefer
Oct 30, 2016·Journal of Molecular and Cellular Cardiology·Yinfang WangPeng Zhang
Dec 4, 2016·The Journal of Endocrinology·Noelia Martínez-SánchezMiguel López
Jan 12, 2017·Tissue Engineering. Part a·Jessica P YangJennifer H Elisseeff
Feb 7, 2017·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·R N PradhanB Deplancke
Jun 15, 2016·PloS One·Katharina LeithnerAndelko Hrzenjak
Feb 17, 2017·Genes & Development·Chelsea HeplerRana K Gupta

❮ Previous
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