Genetic and genome-wide transcriptomic analyses identify co-regulation of oxidative response and hormone transcript abundance with vitamin C content in tomato fruit.

BMC Genomics
Viviana Lima-SilvaMiguel A Botella

Abstract

L-ascorbic acid (AsA; vitamin C) is essential for all living plants where it functions as the main hydrosoluble antioxidant. It has diverse roles in the regulation of plant cell growth and expansion, photosynthesis, and hormone-regulated processes. AsA is also an essential component of the human diet, being tomato fruit one of the main sources of this vitamin. To identify genes responsible for AsA content in tomato fruit, transcriptomic studies followed by clustering analysis were applied to two groups of fruits with contrasting AsA content. These fruits were identified after AsA profiling of an F8 Recombinant Inbred Line (RIL) population generated from a cross between the domesticated species Solanum lycopersicum and the wild relative Solanum pimpinellifollium. We found large variability in AsA content within the RIL population with individual RILs with up to 4-fold difference in AsA content. Transcriptomic analysis identified genes whose expression correlated either positively (PVC genes) or negatively (NVC genes) with the AsA content of the fruits. Cluster analysis using SOTA allowed the identification of subsets of co-regulated genes mainly involved in hormones signaling, such as ethylene, ABA, gibberellin and auxin, rather...Continue Reading

References

Jun 6, 2000·Trends in Plant Science·N HoremansH Asard
Dec 29, 2000·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·N Smirnoff
Apr 20, 2001·Proceedings of the National Academy of Sciences of the United States of America·V G TusherG Chu
Jun 8, 2001·Bioinformatics·O TroyanskayaR B Altman
Oct 13, 2001·Current Opinion in Plant Biology·S Gazzarrini, P McCourt
Mar 5, 2002·Nature·Tsuneaki AsaiJen Sheen
Jun 19, 2002·JAMA : the Journal of the American Medical Association·Kathleen M Fairfield, Robert H Fletcher
Jan 14, 2003·Nature Biotechnology·Fernanda AgiusVictoriano Valpuesta
Mar 5, 2003·BioTechniques·A I SaeedJ Quackenbush
Mar 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Zhong ChenDaniel R Gallie
Mar 21, 2003·Journal of Proteome Research·Javier Herrero, Joaquín Dopazo
Feb 21, 2004·Plant Physiology·Argelia LorenceCraig L Nessler
Sep 18, 2004·Plant Physiology·Philip ZimmermannWilhelm Gruissem
Nov 27, 2004·Trends in Plant Science·Victoriano Valpuesta, Miguel A Botella
Aug 3, 2005·Journal of Experimental Botany·Beata A WoluckaDirk Inzé
Sep 24, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M Cecilia RousseauxAnn Powell
May 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Cornelius S Barry, James J Giovannoni
Aug 8, 2006·Plant Physiology·Zhong Chen, Daniel R Gallie
Apr 20, 2007·Current Opinion in Plant Biology·James J Giovannoni
Jul 10, 2007·Plant Physiology·David Weiss, Naomi Ori
Sep 22, 2007·The Journal of Nutrition·Yi Li, Herb E Schellhorn
Dec 14, 2007·Current Opinion in Plant Biology·Claus Schwechheimer
Feb 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Liviu M Mirica, Judith P Klinman
Aug 16, 2008·The Plant Journal : for Cell and Molecular Biology·Juan Carlos SerraniJosé Luis García-Martínez

❮ Previous
Next ❯

Citations

May 18, 2013·Database : the Journal of Biological Databases and Curation·Anuradha PujarLukas A Mueller
Mar 25, 2014·Journal of Experimental Botany·Adriana BastíasJosé A Casaretto
Apr 20, 2016·Frontiers in Plant Science·Roberta CalafioreAmalia Barone
Oct 6, 2016·International Journal of Molecular Sciences·Erik AlexanderssonErik Andreasson
Jul 27, 2017·Frontiers in Chemistry·Ifigeneia Mellidou, Angelos K Kanellis
Jun 30, 2019·International Journal of Molecular Sciences·Ying WangShiping Tian
May 12, 2017·Frontiers in Plant Science·Nudrat A AkramMuhammad Ashraf
Feb 9, 2019·Frontiers in Plant Science·Mario FenechMiguel A Botella
Jul 16, 2014·BMC Plant Biology·Darren C J WongChristopher M Ford

❮ Previous
Next ❯

Datasets Mentioned

BETA
U586247
U563226

Methods Mentioned

BETA
AsA
High Performance Liquid Chromatography
PCR

Software Mentioned

Prep
Genevestigator
pBase Plus
Blast2GO
GO
Significant Analysis of Microarray ( SAM
TIGR Multiple Experiment Viewer
SAM
AtGenExpress
Excel

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.