Genetic and molecular characteristics of Vir plasmids of bovine septicemic Escherichia coli.

Journal of Bacteriology
J Lopez-AlvarezS Falkow

Abstract

Three wild strains of bovine septicemic Escherichia coli were selected on the basis of their production of a toxin lethal for mice and chickens and their characteristic surface antigen. The transfer of these virulence (Vir) properties from two of the three to recipient E. coli was detected after mating. One Vir plasmid (pJL1) was derepressed for transfer and associated with mobilization of chromosomal markers. The other, pJL2, was repressed. Both plasmids were tagged with transposon Tn5 (kanamycin resistance), and transfer parameters of the tagged plasmids were studied. The Tn5 insertion in pJL2 usually increased transfer efficiency 100-fold. Plasmid pJL1 was classified as a member of the FIV incompatibility group. A pJL1::Tn5 derivative plasmid was incompatible with ColV1. Plasmid pJL2 behaved as an fi+ plasmid. Both plasmids pJL1 and pJL2 had a molecular weight of 92 x 10(6) and were present at about four copies per chromosome; their deoxyribonucleic acid (DNA) structures were not identical on the basis of restriction enzyme analysis. DNA-DNA hybridization revealed a polynucleotide sequence homology of at least 58% between the two plasmids. No plasmids could be detected in one wild or certain laboratory-derived Vir+ E. coli s...Continue Reading

References

Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F HeffronS Falkow
Mar 1, 1978·Journal of Bacteriology·M SoS Falkow
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M L SilvaC L Gyles
May 1, 1978·Infection and Immunity·P L ShipleyS Falkow
Oct 1, 1976·The Journal of Infectious Diseases·R B GrantA J Shapiey
Aug 1, 1975·Infection and Immunity·L P ElwellS Falkow
Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J H CrosaS Falkow
Jan 1, 1972·Journal of Bacteriology·J Stadler, E A Adelberg
Jul 1, 1972·Journal of General Microbiology·R W Hedges, N Datta
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S N CohenL Hsu
Nov 1, 1974·Journal of General Microbiology·S FalkowN Datta
Jun 15, 1974·Journal of Molecular Biology·I ZaenenJ Schell
Jun 1, 1971·Genetical Research·J N Grindley, E S Anderson
Jun 1, 1969·Bacteriological Reviews·D A Marvin, B Hohn

❮ Previous
Next ❯

Citations

Dec 1, 1985·European Journal of Clinical Microbiology·A BraunerG Källenius
Dec 1, 2009·Microbiology and Molecular Biology Reviews : MMBR·Timothy J Johnson, Lisa K Nolan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.