Genetic and molecular properties of an infectious antibiotic resistance (R) factor isolated from Klebsiella.

Journal of Bacteriology
R V Iyer, V N Iyer

Abstract

A Klebsiella strain of human origin that was resistant to ampicillin, chloramphenicol, kanamycin, neomycin, streptomycin, and tetracycline was found to have all of these resistances associated with a R factor and a satellite molecular species of deoxyribonucleic acid (DNA) with an average buoyant density of 1.710 in cesium chloride gradients. There was no evidence of the existence of DNA with other buoyant densities. The strain bears two separable mutations for chloramphenicol resistance, both of which are associated with the R factor (KR9). Exposure of the Klebsiella strain to acridine derivatives or to ethidium bromide (which was more efficient) resulted in partial losses of resistance accompanied by the disappearance of the satellite DNA peak or shifts in its density. The R factor and its component genes were conjugally transmitted across generic boundaries and maintained in new hosts with different efficiencies. The basis of this difference lies not only in the efficiency of conjugal transfer but also in the stability of the components after transfer. All of the resistance genes and the resistance transfer factor were cotransducible by phage Plkc from Escherichia coli. Partially resistant strains could be reconstituted to f...Continue Reading

References

Jun 1, 1966·Journal of Molecular Biology·R RowndA Nakamura
Dec 1, 1968·Journal of General Microbiology·D H BouanchaudY A Chabbert
Feb 1, 1969·Journal of Bacteriology·E Romero, E Meynell
May 1, 1966·Journal of Molecular Biology·S FalkowJ A Wohlhieter
Jan 1, 1969·Journal of Bacteriology·T NisiokaR Clowes
May 27, 1967·Nature·E Meynell, N Datta
Mar 1, 1963·Bacteriological Reviews·T WATANABE
Jul 1, 1962·Journal of Bacteriology·S MITSUHASHIM SUZUKI
Jul 15, 1958·Proceedings of the National Academy of Sciences of the United States of America·M Meselson, F W Stahl

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Citations

Jul 1, 1972·Journal of Bacteriology·S E Russell, V N Iyer
Oct 1, 1972·Journal of Bacteriology·J E Brenchley, B Magasanik
Jan 1, 1972·Journal of Bacteriology·Y Nagai, S Mitsuhashi
Mar 1, 1973·Journal of Theoretical Biology·R R Meyer

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