PMID: 7581374Aug 1, 1995Paper

Genetic and physical characterization of the early-onset Alzheimer's disease AD3 locus on chromosome 14q24.3

Human Molecular Genetics
M CrutsC Van Broeckhoven

Abstract

Genetic linkage studies have provided significant evidence that a major gene defect, AD3, for familial early-onset Alzheimer's disease (EOAD) is located at chromosome 14q24.3, between the short tandem repeat (STR) markers D14S52 and D14S53 defining a genetic size of 22.7 cM for the AD3 candidate region. We constructed a physical map of the AD3 region using yeast artificial chromosomes (YACs) selected from both the CEPH and megaCEPH YAC libraries using the AD3 linked STR markers as well as new sequence-tagged sites (STSs) designed based on YAC terminal sequences. The YAC map is contiguous in the region between D14S258 and D14S53, a region of 8.2 cM, and has an estimated physical size of 4-8 Mb. The YAC contig map was used as a framework to localize three known genes, a pseudogene and two brain expressed sequence tags (ESTs). Linkage analysis studies in two Belgian chromosome 14 EOAD families AD/A and AD/B, identified obligate recombinants in family AD/A with D14S289 and D14S61 reducing the genetic size of the candidate AD3 region substantially. The minimal AD3 candidate region measured 6.4 cM on the genetic map and is contained within six overlapping megaCEPH YACs that covered a physical distance estimated between 2 and 6 Mb. Th...Continue Reading

Citations

Oct 3, 2002·Journal of Neurochemistry·Kazuhiko NamekataHideo Kimura
Feb 12, 2002·Journal of Medical Genetics·D Kamnasaran, D W Cox
Mar 27, 2016·Alzheimer's & Dementia : the Journal of the Alzheimer's Association·Rita CacaceChristine Van Broeckhoven
Mar 26, 1998·American Journal of Medical Genetics·H HouldenJ Hardy
Jan 15, 2019·Journal of Alzheimer's Disease : JAD·Fadia El BitarNada Al Tassan
Aug 24, 2018·Brain : a Journal of Neurology·Vladislav A PetyukAmanda J Myers
Jan 1, 1996·Human Molecular Genetics·M CrutsC Van Broeckhoven
Feb 28, 1998·Genomics·V SharmaG D Schellenberg

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