Genetic and physical mapping of Avr1a in Phytophthora sojae.

Genetics
Terry MacGregorMark Gijzen

Abstract

The interaction between soybean and the phytopathogenic oomycete Phytophthora sojae is controlled by host resistance (Rps) genes and pathogen avirulence (Avr) genes. We have mapped the Avr1a locus in F(2) populations derived from four different P. sojae races. Four RAPD and nine AFLP markers linked to Avr1a were initially identified. Nine markers were used to compare genetic linkage maps of the Avr1a locus in two distinct F(2) populations. Distorted segregation ratios favoring homozygous genotypes were noted in both crosses. Segregation analysis of all the markers in one F(2) population of 90 progeny generated a map of 113.2 cM encompassing Avr1a, with one marker cosegregating with the gene. The cosegregating DNA marker was used to isolate P. sojae BAC clones and construct a physical map covering 170 kb, from which additional DNA markers were developed. Three markers occurring within the BAC contig were mapped in an enlarged population of 486 F(2) progeny. Avr1a was localized to a 114-kb interval, and an average physical to genetic distance ratio of 391 kb/cM was calculated for this region. This work provides a basis for the positional cloning of Avr1a.

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Citations

Apr 10, 2003·Eukaryotic Cell·Sophien Kamoun
Dec 13, 2006·Molecular Plant-microbe Interactions : MPMI·Xuemin ZhangBrett M Tyler
May 14, 2003·Fungal Genetics and Biology : FG & B·Delphine SicardRichard W Michelmore
Dec 4, 2003·Fungal Genetics and Biology : FG & B·Stephen C WhissonAndre Drenth
Nov 2, 2002·The Plant Journal : for Cell and Molecular Biology·Dinah QutobMark Gijzen
Apr 8, 2016·Journal of Agricultural and Food Chemistry·Soroosh Soltani DashtbozorgLu-Kwang Ju
Jul 1, 2003·The New Phytologist·Jorunn I B BosSophien Kamoun
Jan 1, 2007·Molecular Plant Pathology·Brett M Tyler
Mar 3, 2010·Molecular Plant-microbe Interactions : MPMI·Daolong DouBrett M Tyler

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