Genetic and physical mapping of loci for resistance to blackleg disease in canola (Brassica napus L.)

Scientific Reports
Rosy RamanHarsh Raman

Abstract

Sustainable canola production is essential to meet growing human demands for vegetable oil, biodiesel, and meal for stock feed markets. Blackleg, caused by the fungal pathogen, Leptosphaeria maculans is a devastating disease that can lead to significant yield loss in many canola production regions worldwide. Breakdown of race-specific resistance to L. maculans in commercial cultivars poses a constant threat to the canola industry. To identify new alleles, especially for quantitative resistance (QR), we analyzed 177 doubled haploid (DH) lines derived from an RP04/Ag-Outback cross. DH lines were evaluated for QR under field conditions in three experiments conducted at Wagga Wagga (2013, 2014) and Lake Green (2015), and under shade house conditions using the 'ascospore shower' test. DH lines were also characterized for qualitative R gene-mediated resistance via cotyledon tests with two differential single spore isolates, IBCN17 and IBCN76, under glasshouse conditions. Based on 18,851 DArTseq markers, a linkage map representing 2,019 unique marker bins was constructed and then utilized for QTL detection. Marker regression analysis identified 22 significant marker associations for resistance, allowing identification of two race-spec...Continue Reading

References

May 16, 2002·The Journal of Heredity·R E Voorrips
Jun 1, 1997·Genome Génome / Conseil National De Recherches Canada·R MayerhoferG R Stringam
Oct 13, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S KaurG C Spangenberg
Mar 29, 2012·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Rosy RamanHarsh Raman
Apr 5, 2014·Plant Biotechnology Journal·Harsh RamanJacqueline Batley
Jul 29, 2015·Frontiers in Plant Science·Miguel CurtoDiego Rubiales
Nov 1, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Y J HuangR Delourme
May 8, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Vinod KumarRégine Delourme
Dec 12, 2018·Frontiers in Plant Science·Harsh RamanRegine Delourme

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Citations

Jan 6, 2021·International Journal of Molecular Sciences·Aldrin Y CantilaJacqueline Batley
Jun 10, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Junrey AmasJacqueline Batley

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Methods Mentioned

BETA
genotyping

Software Mentioned

DiGGer
Excel
WGAIM
Darmor
BLASTN
asreml
BLASTp
R
ran
Map Chart

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