Genetic and physiological activation of osmosensitive gene expression mimics transcriptional signatures of pathogen infection in C. elegans

PloS One
Anne-Katrin RohlfingTodd Lamitina

Abstract

The soil-dwelling nematode C. elegans is a powerful system for comparative molecular analyses of environmental stress response mechanisms. Infection of worms with bacterial and fungal pathogens causes the activation of well-characterized innate immune transcriptional programs in pathogen-exposed hypodermal and intestinal tissues. However, the pathophysiological events that drive such transcriptional responses are not understood. Here, we show that infection-activated transcriptional responses are, in large part, recapitulated by either physiological or genetic activation of the osmotic stress response. Microarray profiling of wild type worms exposed to non-lethal hypertonicity identified a suite of genes that were also regulated by infection. Expression profiles of five different osmotic stress resistant (osr) mutants under isotonic conditions reiterated the wild type transcriptional response to osmotic stress and also showed substantial similarity to infection-induced gene expression under isotonic conditions. Computational, transgenic, and functional approaches revealed that two GATA transcription factors previously implicated in infection-induced transcriptional responses, elt-2 and elt-3, are also essential for coordinated ...Continue Reading

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Citations

Aug 6, 2010·Molecular Biology of the Cell·Isabelle RothUdo Hasler
May 31, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lorenza E Moronetti MazzeoTodd Lamitina
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Jun 27, 2017·Frontiers in Physiology·Carla Igual GilAnne-Katrin Rohlfing
Aug 12, 2018·Genes·Ana Carolina Remondi SouzaEleftherios Mylonakis
Dec 12, 2020·Developmental Biology·Chee Kiang EweJoel H Rothman

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Datasets Mentioned

BETA
GSE19310

Methods Mentioned

BETA
environmental stress
transgenic
PCR
salt stress
in vitro transcription

Software Mentioned

Partek Genomics Suite
SCAN
Significance analysis of Microarrays ( SAM )
Graphpad Prism
PWM
Affymetrix

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