Genetic and physiological characterization of Escherichia coli mutants deficient in phosphoenolpyruvate carboxykinase activity.

Journal of Bacteriology
A H Goldie, B D Sanwal

Abstract

Mutants doubly deficient in phosphoenolpyruvate carboxykinase (pck) and phosphoenolpyruvate synthetase (pps) were unable to grow with succinate as the sole carbon source. A number of pck mutations isolated from pps strains by penicillin selection mapped at 74 min on the Escherichia coli chromosome, between glpD and aroB. Several of the strains containing these mutations had a protein antigenically related to phosphoenolpyruvate carboxykinase, and therefore, the mutations probably represented mutations in the structural gene for this enzyme. Phosphoenolpyruvate carboxykinase was regulated at the level of transcription by catabolite repression. Enzyme levels also increased in stationary-phase cultures by a mechanism independent of cyclic adenosine monophosphate or the product of the relA gene.

References

Jan 1, 1975·Journal of Bacteriology·A B Deleo, B Magasanik
Jul 22, 1975·Biochemical and Biophysical Research Communications·E J Hansen, E Juni
Jun 1, 1978·Canadian Journal of Biochemistry·W A Bridger, W Paranchych
Jul 28, 1978·Biochemical and Biophysical Research Communications·A H GoldieB D Sanwal
Aug 19, 1974·Biochemical and Biophysical Research Communications·E J Hansen, E Juni
Sep 12, 1967·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·R A Cooper, H L Kornberg
Oct 1, 1972·Analytical Biochemistry·P J Geiger, S P Bessman
Nov 14, 1971·Journal of Molecular Biology·M HofnungD Hatfield
Jul 8, 1965·Biochimica Et Biophysica Acta·R A Cooper, H L Kornberg
Apr 1, 1961·Journal of Molecular Biology·R HAMERS, C HAMERS-CASTERMAN
Feb 26, 1960·Science·L GORINI, H KAUFMAN

❮ Previous
Next ❯

Citations

Apr 11, 2015·Nature Communications·Peter J EastmondJulian M Hibberd
Aug 17, 2005·FEMS Microbiology Reviews·Uwe Sauer, Bernhard J Eikmanns
Feb 18, 2016·Archives of Microbiology·Masaru TamuraAtsushi Kato
Mar 28, 1997·The Journal of Biological Chemistry·A MatteL T Delbaere
Sep 25, 2018·Journal of Medical Microbiology·Jipeng WangJun Zhu
May 10, 2018·Frontiers in Microbiology·Gitanjali NandaKafleVolker S Brözel
Mar 5, 2021·Microbiological Research·Lucas Nojosa OliveiraCélia Maria de Almeida Soares
Jan 1, 1992·Molecular & General Genetics : MGG·M NiersbachH J Hirsch
Aug 30, 2018·Microbial Cell Factories·L KyselovaK Bettenbrock
Dec 1, 2004·EcoSal Plus·Gottfried Unden, Alexandra Kleefeld
Jun 1, 1983·Microbiological Reviews·B J Bachmann
Nov 1, 1992·Journal of Bacteriology·R A Salomón, R N Farías
Dec 1, 1992·Journal of Bacteriology·R PatnaikJ C Liao
Dec 1, 1981·Microbiological Reviews·J L Botsford
Dec 1, 1990·Journal of Bacteriology·N Hugouvieux-Cotte-PattatS Harayama
Nov 1, 2005·EcoSal Plus·Tony Romeo, Jacky L Snoep
Nov 1, 1993·Journal of Bacteriology·Y P ChaoJ C Liao
Aug 1, 1997·Applied and Environmental Microbiology·W R Farmer, J C Liao
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.